Production of IL-8 and release of eosinophil-derived neurotoxin by normal peripheral blood eosinophils

Hirokazu Nakajima, Gerald J. Gleich, Takeshi Fukuda, Sohei Makino, Hirohito Kita

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

To study the regulation of IL-8 production and release of eosinophil-derived neurotoxin (EDN) by normal blood eosinophils, we isolated eosinophils from healthy individuals and stimulated them with immobilized secretory IgA with or without exogenous IL-5 for 3, 12, and 24 h, and with different concentrations of ionomycin for 24 h. Eosinophils cultured with secretory IgA for 3 and 12 h showed strong expression of mRNA for IL-8 by reverse transcription polymerase chain reaction. Exogenous IL-5 enhanced mRNA for IL-8 expression by eosinophils after 24 h of incubation. IL-8 secretion increased in a time-dependent manner throughout the 24 h of observation; in contrast, EDN release reached a plateau value after 12 h. Furthermore, at least 2 μM ionomycin was necessary for induction of IL-8 secretion, whereas 0.5 μM induced detectable EDN release by eosinophils. These results suggest that the mechanism of eosinophil degranulation may be different from those of cytokine secretion.

Original languageEnglish (US)
Pages (from-to)36-39
Number of pages4
JournalInternational Archives of Allergy and Immunology
Volume114
Issue numberSUPPL. 1
StatePublished - Oct 1997

Fingerprint

Eosinophil-Derived Neurotoxin
Interleukin-8
Eosinophils
Secretory Immunoglobulin A
Ionomycin
Interleukin-5
Messenger RNA
Interleukin-3
Reverse Transcription
Observation
Cytokines
Polymerase Chain Reaction

Keywords

  • Eosinophil
  • Eosinophil-derived neurotoxin
  • IgA, secretory
  • IL-8
  • Ionomycin

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Production of IL-8 and release of eosinophil-derived neurotoxin by normal peripheral blood eosinophils. / Nakajima, Hirokazu; Gleich, Gerald J.; Fukuda, Takeshi; Makino, Sohei; Kita, Hirohito.

In: International Archives of Allergy and Immunology, Vol. 114, No. SUPPL. 1, 10.1997, p. 36-39.

Research output: Contribution to journalArticle

Nakajima, Hirokazu ; Gleich, Gerald J. ; Fukuda, Takeshi ; Makino, Sohei ; Kita, Hirohito. / Production of IL-8 and release of eosinophil-derived neurotoxin by normal peripheral blood eosinophils. In: International Archives of Allergy and Immunology. 1997 ; Vol. 114, No. SUPPL. 1. pp. 36-39.
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