Production of IL-8 and Release of Eosinophil-Derived Neurotoxin by Normal Peripheral Blood Eosinophils

Hirokazu Nakajima, Gerald J. Gleich, Takeshi Fukuda, Sohei Makino, Hirohito Kita

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

To study the regulation of IL-8 production and release of eosinophil-derived neurotoxin (EDN) by normal blood eosinophils, we isolated eosinophils from healthy individuals and stimulated them with immobilized secretory IgA with or without exogenous IL-5 for 3, 12, and 24 h, and with different concentrations of ionomycin for 24 h. Eosinophils cultured with secretory IgA for 3 and 12 h showed strong expression of mRNA for IL-8 by reverse transcription polymerase chain reaction. Exogenous IL-5 enhanced mRNA for IL-8 expression by eosinophils after 24 h of incubation. IL-8 secretion increased in a time-dependent manner throughout the 24 h of observation; in contrast, EDN release reached a plateau value after 12 h. Furthermore, at least 2 µM ionomycin was necessary for induction of IL-8 secretion, whereas 0.5 µM induced detectable EDN release by eosinophils. These results suggest that the mechanism of eosinophil degranulation may be different from those of cytokine secretion.

Original languageEnglish (US)
Pages (from-to)36-39
Number of pages4
JournalInternational archives of allergy and immunology
Volume114
Issue number1
DOIs
StatePublished - Jan 1 1997

Keywords

  • Eosinophil
  • Eosinophil-derived neurotoxin
  • IL-8
  • IgA
  • Ionomycin
  • Secretory

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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