TY - JOUR
T1 - Production of erythroid potentiating factor(s) by a human monocytic cell line
AU - Ascensao, J. L.
AU - Kay, N. E.
AU - Earenfight-Engler, T.
AU - Koren, H. S.
AU - Zanjani, E. D.
N1 - Copyright:
Copyright 2020 Elsevier B.V., All rights reserved.
PY - 1981
Y1 - 1981
N2 - U-937 is a human monocytic cell line that has been shown to elaborate factors that affect normal human hematopoiesis in vitro. Studies on the effects of these factors demonstrated an erythroid potentiating factor (EPF) and a potent inhibitor of granulocyte-macrophage (CFU-GM) colony growth. The EPF was present in both serum-containing and serum-free U-937 conditioned media, had a dose-dependent effect on erythroid colony formation and was remarkably heat stable. The CFU-GM inhibitory activity was also detected in serum-free conditioned medium, was dose-dependent, heat labile and its effect was reversed by indomethacin. Indomethacin (Sigma, St. Louis, Mo.) did not alter the erythroid effects of the U-937 conditioned medium. No colony stimulating factor (CSF) or erythropoietin (Ep) could be detected in this medium. The existence of a human cell line capable of producing EPF without simultaneous CSF production will permit further studies on the biochemical and biologic nature of these factors.
AB - U-937 is a human monocytic cell line that has been shown to elaborate factors that affect normal human hematopoiesis in vitro. Studies on the effects of these factors demonstrated an erythroid potentiating factor (EPF) and a potent inhibitor of granulocyte-macrophage (CFU-GM) colony growth. The EPF was present in both serum-containing and serum-free U-937 conditioned media, had a dose-dependent effect on erythroid colony formation and was remarkably heat stable. The CFU-GM inhibitory activity was also detected in serum-free conditioned medium, was dose-dependent, heat labile and its effect was reversed by indomethacin. Indomethacin (Sigma, St. Louis, Mo.) did not alter the erythroid effects of the U-937 conditioned medium. No colony stimulating factor (CSF) or erythropoietin (Ep) could be detected in this medium. The existence of a human cell line capable of producing EPF without simultaneous CSF production will permit further studies on the biochemical and biologic nature of these factors.
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U2 - 10.1182/blood.v57.1.170.bloodjournal571170
DO - 10.1182/blood.v57.1.170.bloodjournal571170
M3 - Article
C2 - 6969611
AN - SCOPUS:0019378082
VL - 57
SP - 170
EP - 173
JO - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
JF - American Journal of Physiology - Renal Fluid and Electrolyte Physiology
SN - 1931-857X
IS - 1
ER -