Production, harvest, and concentration of feline immunodeficiency virus-based lentiviral vector from cells grown in CF10 or CF2 devices

Dyana T. Saenz, Román Barraza, Nils Loewen, Wulin Teo, Eric M. Poeschla

Research output: Contribution to journalArticlepeer-review

6 Scopus citations

Abstract

Feline immunodeficiency virus (FIV)-based lentiviral vectors are useful for introducing integrated transgenes into nondividing human cells. This protocol describes the production of FIV-based lentiviral vectors using cells grown in CF10 or CF2 devices. It also details the harvesting and concentration of these vectors. The methods are for production for basic science laboratory use and in vivo experimentation. They do not meet standards for clinical-grade (good manufacturing practice [GMP]) production.

Original languageEnglish (US)
Pages (from-to)118-123
Number of pages6
JournalCold Spring Harbor Protocols
Volume7
Issue number1
DOIs
StatePublished - Jan 2012

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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