Production and harvest of feline immunodeficiency virus-based lentiviral vector from cells grown in T75 tissue-culture flasks

Dyana T. Saenz, Román Barraza, Nils Loewen, Wulin Teo, Eric M. Poeschla

Research output: Contribution to journalArticle

5 Scopus citations

Abstract

Feline immunodeficiency virus (FIV)-based lentiviral vectors are useful for introducing integrated transgenes into nondividing human cells. This protocol describes the production and harvesting of vector from cells grown in T75 tissue-culture flasks. The methods are for production for basic science laboratory use and in vivo experimentation. They do not meet standards for clinical-grade (good manufacturing practice [GMP]) production.

Original languageEnglish (US)
Pages (from-to)124-125
Number of pages2
JournalCold Spring Harbor Protocols
Volume7
Issue number1
DOIs
StatePublished - Jan 2012
Externally publishedYes

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ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

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