Production and harvest of feline immunodeficiency virus-based lentiviral vector from cells grown in T75 tissue-culture flasks

Dyana T. Saenz; Román Barraza; Nils Loewen; Wulin Teo; Eric M. Poeschla

doi:10.1101/pdb.prot067553

Production and harvest of feline immunodeficiency virus-based lentiviral vector from cells grown in T75 tissue-culture flasks

Dyana T. Saenz, Román Barraza, Nils Loewen, Wulin Teo, Eric M. Poeschla

Molecular Medicine

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Feline immunodeficiency virus (FIV)-based lentiviral vectors are useful for introducing integrated transgenes into nondividing human cells. This protocol describes the production and harvesting of vector from cells grown in T75 tissue-culture flasks. The methods are for production for basic science laboratory use and in vivo experimentation. They do not meet standards for clinical-grade (good manufacturing practice [GMP]) production.

Original language	English (US)
Pages (from-to)	124-125
Number of pages	2
Journal	Cold Spring Harbor Protocols
Volume	7
Issue number	1
DOIs	https://doi.org/10.1101/pdb.prot067553
State	Published - Jan 2012

ASJC Scopus subject areas

Biochemistry, Genetics and Molecular Biology(all)

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10.1101/pdb.prot067553

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abstract = "Feline immunodeficiency virus (FIV)-based lentiviral vectors are useful for introducing integrated transgenes into nondividing human cells. This protocol describes the production and harvesting of vector from cells grown in T75 tissue-culture flasks. The methods are for production for basic science laboratory use and in vivo experimentation. They do not meet standards for clinical-grade (good manufacturing practice [GMP]) production.",

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AU - Teo, Wulin

AU - Poeschla, Eric M.

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Production and harvest of feline immunodeficiency virus-based lentiviral vector from cells grown in T75 tissue-culture flasks

Abstract

ASJC Scopus subject areas

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