Production and characterization of monoclonal antibodies to human sclerostin

Theodore A. Craig, Stacy L. Sommer, Thomas G. Beito, Rajiv Kumar

Research output: Contribution to journalArticlepeer-review

4 Scopus citations

Abstract

We developed and characterized monoclonal antibodies directed against the amino-terminal and carboxy-terminal regions of human and mouse sclerostin (scl). Amino-terminal and carboxy-terminal scl peptides with limited homology to scl domain-containing protein-1 were synthesized using f-moc chemistry. The peptides were conjugated to keyhole limpet hemocyanin and the conjugates were used for immunization of mice. Monoclonal antibodies were obtained and characterized using bacterially expressed and insect cell-expressed recombinant scl. The amino-terminal (IgG 2aK) and carboxy-terminal (IgG 2bK) antibodies bound bioactive sclerostin that was expressed in an insect-cell expression system with dissociation constants in the nanomolar range. The antibodies are potentially useful agents that can be used for modulating sclerostin bioactivity.

Original languageEnglish (US)
Pages (from-to)377-381
Number of pages5
JournalHybridoma
Volume28
Issue number5
DOIs
StatePublished - Oct 1 2009

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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