Abstract
We developed and characterized monoclonal antibodies directed against the amino-terminal and carboxy-terminal regions of human and mouse sclerostin (scl). Amino-terminal and carboxy-terminal scl peptides with limited homology to scl domain-containing protein-1 were synthesized using f-moc chemistry. The peptides were conjugated to keyhole limpet hemocyanin and the conjugates were used for immunization of mice. Monoclonal antibodies were obtained and characterized using bacterially expressed and insect cell-expressed recombinant scl. The amino-terminal (IgG 2aK) and carboxy-terminal (IgG 2bK) antibodies bound bioactive sclerostin that was expressed in an insect-cell expression system with dissociation constants in the nanomolar range. The antibodies are potentially useful agents that can be used for modulating sclerostin bioactivity.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 377-381 |
| Number of pages | 5 |
| Journal | Hybridoma |
| Volume | 28 |
| Issue number | 5 |
| DOIs | |
| State | Published - Oct 1 2009 |
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ASJC Scopus subject areas
- Immunology
- Immunology and Allergy
Cite this
Production and characterization of monoclonal antibodies to human sclerostin. / Craig, Theodore A.; Sommer, Stacy L.; Beito, Thomas G.; Kumar, Rajiv.
In: Hybridoma, Vol. 28, No. 5, 01.10.2009, p. 377-381.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Production and characterization of monoclonal antibodies to human sclerostin
AU - Craig, Theodore A.
AU - Sommer, Stacy L.
AU - Beito, Thomas G.
AU - Kumar, Rajiv
PY - 2009/10/1
Y1 - 2009/10/1
N2 - We developed and characterized monoclonal antibodies directed against the amino-terminal and carboxy-terminal regions of human and mouse sclerostin (scl). Amino-terminal and carboxy-terminal scl peptides with limited homology to scl domain-containing protein-1 were synthesized using f-moc chemistry. The peptides were conjugated to keyhole limpet hemocyanin and the conjugates were used for immunization of mice. Monoclonal antibodies were obtained and characterized using bacterially expressed and insect cell-expressed recombinant scl. The amino-terminal (IgG 2aK) and carboxy-terminal (IgG 2bK) antibodies bound bioactive sclerostin that was expressed in an insect-cell expression system with dissociation constants in the nanomolar range. The antibodies are potentially useful agents that can be used for modulating sclerostin bioactivity.
AB - We developed and characterized monoclonal antibodies directed against the amino-terminal and carboxy-terminal regions of human and mouse sclerostin (scl). Amino-terminal and carboxy-terminal scl peptides with limited homology to scl domain-containing protein-1 were synthesized using f-moc chemistry. The peptides were conjugated to keyhole limpet hemocyanin and the conjugates were used for immunization of mice. Monoclonal antibodies were obtained and characterized using bacterially expressed and insect cell-expressed recombinant scl. The amino-terminal (IgG 2aK) and carboxy-terminal (IgG 2bK) antibodies bound bioactive sclerostin that was expressed in an insect-cell expression system with dissociation constants in the nanomolar range. The antibodies are potentially useful agents that can be used for modulating sclerostin bioactivity.
UR - http://www.scopus.com/inward/record.url?scp=70350438265&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=70350438265&partnerID=8YFLogxK
U2 - 10.1089/hyb.2009.0036
DO - 10.1089/hyb.2009.0036
M3 - Article
C2 - 19857121
AN - SCOPUS:70350438265
VL - 28
SP - 377
EP - 381
JO - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
JF - Monoclonal Antibodies in Immunodiagnosis and Immunotherapy
SN - 2167-9436
IS - 5
ER -