Probing morbillivirus antisera neutralization using functional chimerism between measles virus and canine distemper virus envelope glycoproteins

Miguel Angel Muñoz-Alía, Stephen J Russell

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Measles virus (MeV) is monotypic. Live virus challenge provokes a broadly protective humoral immune response that neutralizes all known measles genotypes. The two surface glycoproteins, H and F, mediate virus attachment and entry, respectively, and neutralizing antibodies to H are considered the main correlate of protection. Herein, we made improvements to the MeV reverse genetics system and generated a panel of recombinant MeVs in which the globular head domain or stalk region of the H glycoprotein or the entire F protein, or both, were substituted with the corresponding protein domains from canine distemper virus (CDV), a closely related morbillivirus that resists neutralization by measles-immune sera. The viruses were tested for sensitivity to human or guinea pig neutralizing anti-MeV antisera and to ferret anti-CDV antisera. Virus neutralization was mediated by antibodies to both H and F proteins, with H being immunodominant in the case of MeV and F being so in the case of CDV. Additionally, the globular head domains of both MeV and CDV H proteins were immunodominant over their stalk regions. These data shed further light on the factors constraining the evolution of new morbillivirus serotypes.

Original languageEnglish (US)
Article number688
JournalViruses
Volume11
Issue number8
DOIs
StatePublished - Aug 1 2019

Fingerprint

Morbillivirus
Canine Distemper Virus
Measles virus
Chimerism
Immune Sera
Glycoproteins
Measles
Viruses
Virus Attachment
Reverse Genetics
Virus Internalization
Ferrets
Proteins
Membrane Glycoproteins
Humoral Immunity
Neutralizing Antibodies
Guinea Pigs
Genotype
Antibodies

Keywords

  • Chimeras
  • Fusion protein
  • Hemagglutinin protein
  • Neutralizing antibodies
  • Paramyxovirus
  • Vaccines

ASJC Scopus subject areas

  • Infectious Diseases
  • Virology

Cite this

Probing morbillivirus antisera neutralization using functional chimerism between measles virus and canine distemper virus envelope glycoproteins. / Muñoz-Alía, Miguel Angel; Russell, Stephen J.

In: Viruses, Vol. 11, No. 8, 688, 01.08.2019.

Research output: Contribution to journalArticle

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