Probable role of amphiphilicity in the binding of mastoparan to calmodulin

Lynda McDowell, Gautam Sanyal, Franklyn G. Prendergast

Research output: Contribution to journalArticle

60 Citations (Scopus)

Abstract

Two-dimensional helical wheel diagrams and calculations of mean hydrophobic moments show mastoparan, mastoparan X, and Polistes mastoparan to have all the properties expected for amphiphilic helices. Circular dichroic properties are consistent with a random form for these peptides in dilute aqueous solution, but >50% helix is apparent when the peptides are dissolved in 70% trifluoroethanol/water mixtures (v/v) or when the peptides are bound to calmodulin. Changes in the fluorescence spectra, anisotropy, and accessibility of tryptophan whose indole side chain is on the apolar surface of the amphiphilic helix imply a significant role for the apolar surface in the binding of the mastoparans and another amphiphilic peptide, melittin, to calmodulin. These data provide a useful model for designing high-affinity synthetic peptide inhibitors of calmodulin.

Original languageEnglish (US)
Pages (from-to)2979-2984
Number of pages6
JournalBiochemistry
Volume24
Issue number12
StatePublished - 1985

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Calmodulin
Peptides
Trifluoroethanol
Melitten
Fluorescence Polarization
Tryptophan
Wheels
Anisotropy
Fluorescence
mastoparan
Water

ASJC Scopus subject areas

  • Biochemistry

Cite this

McDowell, L., Sanyal, G., & Prendergast, F. G. (1985). Probable role of amphiphilicity in the binding of mastoparan to calmodulin. Biochemistry, 24(12), 2979-2984.

Probable role of amphiphilicity in the binding of mastoparan to calmodulin. / McDowell, Lynda; Sanyal, Gautam; Prendergast, Franklyn G.

In: Biochemistry, Vol. 24, No. 12, 1985, p. 2979-2984.

Research output: Contribution to journalArticle

McDowell, L, Sanyal, G & Prendergast, FG 1985, 'Probable role of amphiphilicity in the binding of mastoparan to calmodulin', Biochemistry, vol. 24, no. 12, pp. 2979-2984.
McDowell L, Sanyal G, Prendergast FG. Probable role of amphiphilicity in the binding of mastoparan to calmodulin. Biochemistry. 1985;24(12):2979-2984.
McDowell, Lynda ; Sanyal, Gautam ; Prendergast, Franklyn G. / Probable role of amphiphilicity in the binding of mastoparan to calmodulin. In: Biochemistry. 1985 ; Vol. 24, No. 12. pp. 2979-2984.
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