Primer on medical genomics. Part II: Background principles and methods in molecular genetics

Ayalew Tefferi, Eric D Wieben, Gordon W. Dewald, David A H Whiteman, Matthew E. Bernard, Thomas C. Spelsberg

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The nucleus of every human cell contains the full complement of the human genome, which consists of approximately 30,000 to 70,000 named and unnamed genes and many intergenic DNA sequences. The double-helical DNA molecule in a human cell, associated with special proteins, is highly compacted into 22 pairs of autosomal chromosomes and an additional pair of sex chromosomes. The entire cellular DNA consists of approximately 3 billion base pairs, of which only 1% is thought to encode a functional protein or a polypeptide. Genetic information is expressed and regulated through a complex system of DNA transcription, RNA processing, RNA translation, and posttranslational and cotranslational modification of proteins. Advances in molecular biology techniques have allowed accurate and rapid characterization of DNA sequences as well as identification and quantification of cellular RNA and protein. Global analytic methods and human genetic mapping are expected to accelerate the process of identification and localization of disease genes. In this second part of an educational series in medical genomics, selected principles and methods in molecular biology are recapped, with the intent to prepare the reader for forthcoming articles with a more direct focus on aspects of the subject matter.

Original languageEnglish (US)
Pages (from-to)785-808
Number of pages24
JournalMayo Clinic Proceedings
Volume77
Issue number8
StatePublished - 2002

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Genomics
Molecular Biology
Intergenic DNA
RNA
DNA
Proteins
Sex Chromosomes
Medical Genetics
Human Genome
Post Translational Protein Processing
Base Pairing
Genes
Chromosomes
Peptides

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Tefferi, A., Wieben, E. D., Dewald, G. W., Whiteman, D. A. H., Bernard, M. E., & Spelsberg, T. C. (2002). Primer on medical genomics. Part II: Background principles and methods in molecular genetics. Mayo Clinic Proceedings, 77(8), 785-808.

Primer on medical genomics. Part II : Background principles and methods in molecular genetics. / Tefferi, Ayalew; Wieben, Eric D; Dewald, Gordon W.; Whiteman, David A H; Bernard, Matthew E.; Spelsberg, Thomas C.

In: Mayo Clinic Proceedings, Vol. 77, No. 8, 2002, p. 785-808.

Research output: Contribution to journalArticle

Tefferi, A, Wieben, ED, Dewald, GW, Whiteman, DAH, Bernard, ME & Spelsberg, TC 2002, 'Primer on medical genomics. Part II: Background principles and methods in molecular genetics', Mayo Clinic Proceedings, vol. 77, no. 8, pp. 785-808.
Tefferi, Ayalew ; Wieben, Eric D ; Dewald, Gordon W. ; Whiteman, David A H ; Bernard, Matthew E. ; Spelsberg, Thomas C. / Primer on medical genomics. Part II : Background principles and methods in molecular genetics. In: Mayo Clinic Proceedings. 2002 ; Vol. 77, No. 8. pp. 785-808.
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