Primary structure of the Aequorea victoria green-fluorescent protein

Douglas C. Prasher, Virginia K. Eckenrode, William W. Ward, Frank G. Prendergast, Milton J. Cormier

Research output: Contribution to journalArticle

1621 Citations (Scopus)

Abstract

Many cnidarians utilize green-fluorescent proteins (GFPs) as energy-transfer acceptors in bioluminescence. GFPs fluoresce in vivo upon receiving energy from either a luciferase-oxyluciferin excited-state complex or a Ca2+-activated photoprotein. These highly fluorescent proteins are unique due to the chemical nature of their chromophore, which is comprised of modified amino acid (aa) residues within the polypeptide. This report describes the cloning and sequencing of both cDNA and genomic clones of GFP from the cnidarian, Aequorea victoria. The gfp10 cDNA encodes a 238-aa-residue polypeptide with a calculated Mr of 26888. Comparsion of A. victoria GFP genomic clones shows three different restriction enzyme patterns which suggests that at least three different genes are present in the A. victoria population at Friday Harbor, Washington. The gfp gene encoded by the λGFP2 genomic clone is comprised of at least three exons spread over 2.6 kb. The nucleotide sequences of the cDNA and the gene will aid in the elucidation of structure-function relationships in this unique class of proteins.

Original languageEnglish (US)
Pages (from-to)229-233
Number of pages5
JournalGene
Volume111
Issue number2
DOIs
StatePublished - Feb 15 1992

Fingerprint

Green Fluorescent Proteins
Cnidaria
Complementary DNA
Clone Cells
Luminescent Proteins
Genes
Amino Acids
Peptides
Energy Transfer
Luciferases
Organism Cloning
Exons
Proteins
Enzymes
Population
Aequorea victoria green fluorescent protein

Keywords

  • aequorin
  • Bioluminescence
  • chromophore
  • cloning
  • Cnidaria
  • energy transfer

ASJC Scopus subject areas

  • Genetics

Cite this

Prasher, D. C., Eckenrode, V. K., Ward, W. W., Prendergast, F. G., & Cormier, M. J. (1992). Primary structure of the Aequorea victoria green-fluorescent protein. Gene, 111(2), 229-233. https://doi.org/10.1016/0378-1119(92)90691-H

Primary structure of the Aequorea victoria green-fluorescent protein. / Prasher, Douglas C.; Eckenrode, Virginia K.; Ward, William W.; Prendergast, Frank G.; Cormier, Milton J.

In: Gene, Vol. 111, No. 2, 15.02.1992, p. 229-233.

Research output: Contribution to journalArticle

Prasher, DC, Eckenrode, VK, Ward, WW, Prendergast, FG & Cormier, MJ 1992, 'Primary structure of the Aequorea victoria green-fluorescent protein', Gene, vol. 111, no. 2, pp. 229-233. https://doi.org/10.1016/0378-1119(92)90691-H
Prasher DC, Eckenrode VK, Ward WW, Prendergast FG, Cormier MJ. Primary structure of the Aequorea victoria green-fluorescent protein. Gene. 1992 Feb 15;111(2):229-233. https://doi.org/10.1016/0378-1119(92)90691-H
Prasher, Douglas C. ; Eckenrode, Virginia K. ; Ward, William W. ; Prendergast, Frank G. ; Cormier, Milton J. / Primary structure of the Aequorea victoria green-fluorescent protein. In: Gene. 1992 ; Vol. 111, No. 2. pp. 229-233.
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