Primary structure of human plasma membrane Ca2+-ATPase isoform 3

Billie Jo Brown; Helene Hilfiker; Steven J. DeMarco; David A. Zacharias; Tammy M. Greenwood; Danilo Guerini; Emanuel E. Strehler

doi:10.1016/0005-2736(96)00108-3

Primary structure of human plasma membrane Ca²⁺-ATPase isoform 3

Billie Jo Brown, Helene Hilfiker, Steven J. DeMarco, David A. Zacharias, Tammy M. Greenwood, Danilo Guerini, Emanuel E. Strehler

Biochemistry and Molecular Biology

Research output: Contribution to journal › Article › peer-review

18 Scopus citations

Abstract

The complete coding sequence of the human plasma membrane calcium ATPase (PMCA) isoform 3 was determined from overlapping genomic and cDNA clones. The cDNAs for the two major alternative splice variants 3a (3CII) and 3b (3CI) code for proteins of 1173 and 1220 amino-acid residues, respectively, which show 98% identity with the corresponding rat isoforms. On a multiple human tissue Northern blot, a major PMCA3 transcript of about 7 kb was detected exclusively in the brain, demonstrating the highly restricted pattern of expression of this isoform to human neuronal tissues. With the elucidation of the human PMCA3 primary structure, complete sequence information is now available for the entire family of human PMCA isoforms.

Original language	English (US)
Pages (from-to)	10-13
Number of pages	4
Journal	Biochimica et Biophysica Acta - Biomembranes
Volume	1283
Issue number	1
DOIs	https://doi.org/10.1016/0005-2736(96)00108-3
State	Published - Aug 14 1996

Keywords

ATPase, Ca-
Calcium pump
Human
Isoform
Plasma membrane

ASJC Scopus subject areas

Biophysics
Biochemistry
Cell Biology

Access to Document

10.1016/0005-2736(96)00108-3

Cite this

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title = "Primary structure of human plasma membrane Ca2+-ATPase isoform 3",

abstract = "The complete coding sequence of the human plasma membrane calcium ATPase (PMCA) isoform 3 was determined from overlapping genomic and cDNA clones. The cDNAs for the two major alternative splice variants 3a (3CII) and 3b (3CI) code for proteins of 1173 and 1220 amino-acid residues, respectively, which show 98% identity with the corresponding rat isoforms. On a multiple human tissue Northern blot, a major PMCA3 transcript of about 7 kb was detected exclusively in the brain, demonstrating the highly restricted pattern of expression of this isoform to human neuronal tissues. With the elucidation of the human PMCA3 primary structure, complete sequence information is now available for the entire family of human PMCA isoforms.",

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AU - Brown, Billie Jo

AU - Hilfiker, Helene

AU - DeMarco, Steven J.

AU - Zacharias, David A.

AU - Greenwood, Tammy M.

AU - Guerini, Danilo

AU - Strehler, Emanuel E.

PY - 1996/8/14

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