Primary structure of human plasma membrane Ca2+-ATPase isoform 3

Billie Jo Brown, Helene Hilfiker, Steven J. DeMarco, David A. Zacharias, Tammy M. Greenwood, Danilo Guerini, Emanuel E. Strehler

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

The complete coding sequence of the human plasma membrane calcium ATPase (PMCA) isoform 3 was determined from overlapping genomic and cDNA clones. The cDNAs for the two major alternative splice variants 3a (3CII) and 3b (3CI) code for proteins of 1173 and 1220 amino-acid residues, respectively, which show 98% identity with the corresponding rat isoforms. On a multiple human tissue Northern blot, a major PMCA3 transcript of about 7 kb was detected exclusively in the brain, demonstrating the highly restricted pattern of expression of this isoform to human neuronal tissues. With the elucidation of the human PMCA3 primary structure, complete sequence information is now available for the entire family of human PMCA isoforms.

Original languageEnglish (US)
Pages (from-to)10-13
Number of pages4
JournalBiochimica et Biophysica Acta - Biomembranes
Volume1283
Issue number1
DOIs
StatePublished - Aug 14 1996

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Calcium-Transporting ATPases
Cell membranes
Plasma Membrane Calcium-Transporting ATPases
Protein Isoforms
Cell Membrane
Complementary DNA
Tissue
Rats
Brain
Northern Blotting
Amino Acids
Clone Cells
Proteins

Keywords

  • ATPase, Ca-
  • Calcium pump
  • Human
  • Isoform
  • Plasma membrane

ASJC Scopus subject areas

  • Biochemistry
  • Cell Biology
  • Biophysics

Cite this

Brown, B. J., Hilfiker, H., DeMarco, S. J., Zacharias, D. A., Greenwood, T. M., Guerini, D., & Strehler, E. E. (1996). Primary structure of human plasma membrane Ca2+-ATPase isoform 3. Biochimica et Biophysica Acta - Biomembranes, 1283(1), 10-13. https://doi.org/10.1016/0005-2736(96)00108-3

Primary structure of human plasma membrane Ca2+-ATPase isoform 3. / Brown, Billie Jo; Hilfiker, Helene; DeMarco, Steven J.; Zacharias, David A.; Greenwood, Tammy M.; Guerini, Danilo; Strehler, Emanuel E.

In: Biochimica et Biophysica Acta - Biomembranes, Vol. 1283, No. 1, 14.08.1996, p. 10-13.

Research output: Contribution to journalArticle

Brown, BJ, Hilfiker, H, DeMarco, SJ, Zacharias, DA, Greenwood, TM, Guerini, D & Strehler, EE 1996, 'Primary structure of human plasma membrane Ca2+-ATPase isoform 3', Biochimica et Biophysica Acta - Biomembranes, vol. 1283, no. 1, pp. 10-13. https://doi.org/10.1016/0005-2736(96)00108-3
Brown BJ, Hilfiker H, DeMarco SJ, Zacharias DA, Greenwood TM, Guerini D et al. Primary structure of human plasma membrane Ca2+-ATPase isoform 3. Biochimica et Biophysica Acta - Biomembranes. 1996 Aug 14;1283(1):10-13. https://doi.org/10.1016/0005-2736(96)00108-3
Brown, Billie Jo ; Hilfiker, Helene ; DeMarco, Steven J. ; Zacharias, David A. ; Greenwood, Tammy M. ; Guerini, Danilo ; Strehler, Emanuel E. / Primary structure of human plasma membrane Ca2+-ATPase isoform 3. In: Biochimica et Biophysica Acta - Biomembranes. 1996 ; Vol. 1283, No. 1. pp. 10-13.
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