Primary culture of rat hepatocytes entrapped in cylindrical collagen gels: An in vitro system with application to the bioartificial liver - Rat hepatocytes cultured in cylindrical collagen gels

Scott L. Nyberg, Russell A. Shatford, William D. Payne, Wei Shou Hu, Frank B. Cerra

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

A static culture model employing cylindrical collagen-hepatocyte gels is reported for large scale testing of conditions relevant to the three compartment hollow fiber bioartificial liver. High density hepatocyte cultivation was achieved by cell entrapment within the collagen-hepatocyte gel. Hepatocyte viability was assessed by vital staining, gel contraction, and insulin utilization. Measures of hepatocyte-specific function included albumin synthesis, ureagenesis, lidocaine biotransformation, and cholate conjugation. Although hepatocyte viability remained stable through the seven day incubation period, hepatocyte functions were not uniformly preserved. Albumin synthesis remained stable, while representative P-450 and conjugation activities decreased with time. This static culture system will facilitate the development of a hollow fiber bioartificial liver which utilizes cylindrical collagen-hepatocyte gels.

Original languageEnglish (US)
Pages (from-to)205-215
Number of pages11
JournalCytotechnology
Volume10
Issue number3
DOIs
StatePublished - Jan 1 1992

Keywords

  • artificial organ
  • bioartificial liver
  • gel entrapment
  • hepatocyte
  • metabolism

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Biomedical Engineering
  • Clinical Biochemistry
  • Cell Biology

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