Prevalence of TT virus infection in blood donors with elevated ALT in the absence of known hepatitis markers

Peter J. Cleavinger, David H. Persing, Haijing Li, S. Breanndan Moore, Michael R. Charlton, Corey Sievers, Terry M Therneau, Nizar N. Zein

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

OBJECTIVE: Recently a novel DNA virus (TT virus) has been identified in Japan and shown to be associated with elevated aminotransferase levels after blood transfusion. The exact role of TTV in the pathogenesis of liver disease is yet to be established. Our aim was to determine the prevalence and role of TTV in the pathogenesis of elevated transaminases in healthy blood donors in the absence of markers for viral hepatitis A-C. METHODS: Stored sera were collected from 99 healthy blood donors with elevated alanine amino transferase (ALT) values that were discovered at the time of blood donation. A total of 146 samples were obtained from healthy donors with normal ALT values who were used as controls. None of the patients or controls had a history of blood transfusion or had clinical signs of acute or chronic hepatitis. Serological markers for hepatitis A, hepatitis B, and hepatitis C viruses were negative. TTV DNA was amplified and detected using polymerase chain reaction followed by gel electrophoresis. RESULTS: Five of 99 (5%) samples obtained from donors with elevated ALT had TTV DNA detected by PCR, as compared to one of 146 (0.7%) of those with normal ALT (p = 0.006). Among those with elevated ALT, mean ALT values in patients with TTV (296 ± 305 U/L) were higher than in patients without TTV (95 ± 37 U/L), but the difference was not statistically significant (p = 0.08). The two samples with highest ALT values (both >450 U/L) were among the five samples with detectable TTV DNA in serum. CONCLUSIONS: Although TTV is not likely to explain the majority of elevated ALT cases in otherwise healthy blood donors, TTV infection may potentially be associated with some cases. Based on these findings, we propose that the role of TTV in the pathogenesis of acute and chronic liver diseases merits further investigation. (C) 2000 by Am. Coll. of Gastroenterology.

Original languageEnglish (US)
Pages (from-to)772-776
Number of pages5
JournalAmerican Journal of Gastroenterology
Volume95
Issue number3
DOIs
StatePublished - Mar 2000

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Torque teno virus
Virus Diseases
Transferases
Blood Donors
Alanine
Hepatitis
Hepatitis A
Transaminases
Blood Transfusion
Liver Diseases
DNA
Tissue Donors
Polymerase Chain Reaction
DNA Viruses
Gastroenterology
Chronic Hepatitis
Hepatitis C
Hepatitis B
Serum
Hepacivirus

ASJC Scopus subject areas

  • Gastroenterology

Cite this

Cleavinger, P. J., Persing, D. H., Li, H., Moore, S. B., Charlton, M. R., Sievers, C., ... Zein, N. N. (2000). Prevalence of TT virus infection in blood donors with elevated ALT in the absence of known hepatitis markers. American Journal of Gastroenterology, 95(3), 772-776. https://doi.org/10.1016/S0002-9270(99)00879-5

Prevalence of TT virus infection in blood donors with elevated ALT in the absence of known hepatitis markers. / Cleavinger, Peter J.; Persing, David H.; Li, Haijing; Moore, S. Breanndan; Charlton, Michael R.; Sievers, Corey; Therneau, Terry M; Zein, Nizar N.

In: American Journal of Gastroenterology, Vol. 95, No. 3, 03.2000, p. 772-776.

Research output: Contribution to journalArticle

Cleavinger, Peter J. ; Persing, David H. ; Li, Haijing ; Moore, S. Breanndan ; Charlton, Michael R. ; Sievers, Corey ; Therneau, Terry M ; Zein, Nizar N. / Prevalence of TT virus infection in blood donors with elevated ALT in the absence of known hepatitis markers. In: American Journal of Gastroenterology. 2000 ; Vol. 95, No. 3. pp. 772-776.
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abstract = "OBJECTIVE: Recently a novel DNA virus (TT virus) has been identified in Japan and shown to be associated with elevated aminotransferase levels after blood transfusion. The exact role of TTV in the pathogenesis of liver disease is yet to be established. Our aim was to determine the prevalence and role of TTV in the pathogenesis of elevated transaminases in healthy blood donors in the absence of markers for viral hepatitis A-C. METHODS: Stored sera were collected from 99 healthy blood donors with elevated alanine amino transferase (ALT) values that were discovered at the time of blood donation. A total of 146 samples were obtained from healthy donors with normal ALT values who were used as controls. None of the patients or controls had a history of blood transfusion or had clinical signs of acute or chronic hepatitis. Serological markers for hepatitis A, hepatitis B, and hepatitis C viruses were negative. TTV DNA was amplified and detected using polymerase chain reaction followed by gel electrophoresis. RESULTS: Five of 99 (5{\%}) samples obtained from donors with elevated ALT had TTV DNA detected by PCR, as compared to one of 146 (0.7{\%}) of those with normal ALT (p = 0.006). Among those with elevated ALT, mean ALT values in patients with TTV (296 ± 305 U/L) were higher than in patients without TTV (95 ± 37 U/L), but the difference was not statistically significant (p = 0.08). The two samples with highest ALT values (both >450 U/L) were among the five samples with detectable TTV DNA in serum. CONCLUSIONS: Although TTV is not likely to explain the majority of elevated ALT cases in otherwise healthy blood donors, TTV infection may potentially be associated with some cases. Based on these findings, we propose that the role of TTV in the pathogenesis of acute and chronic liver diseases merits further investigation. (C) 2000 by Am. Coll. of Gastroenterology.",
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T1 - Prevalence of TT virus infection in blood donors with elevated ALT in the absence of known hepatitis markers

AU - Cleavinger, Peter J.

AU - Persing, David H.

AU - Li, Haijing

AU - Moore, S. Breanndan

AU - Charlton, Michael R.

AU - Sievers, Corey

AU - Therneau, Terry M

AU - Zein, Nizar N.

PY - 2000/3

Y1 - 2000/3

N2 - OBJECTIVE: Recently a novel DNA virus (TT virus) has been identified in Japan and shown to be associated with elevated aminotransferase levels after blood transfusion. The exact role of TTV in the pathogenesis of liver disease is yet to be established. Our aim was to determine the prevalence and role of TTV in the pathogenesis of elevated transaminases in healthy blood donors in the absence of markers for viral hepatitis A-C. METHODS: Stored sera were collected from 99 healthy blood donors with elevated alanine amino transferase (ALT) values that were discovered at the time of blood donation. A total of 146 samples were obtained from healthy donors with normal ALT values who were used as controls. None of the patients or controls had a history of blood transfusion or had clinical signs of acute or chronic hepatitis. Serological markers for hepatitis A, hepatitis B, and hepatitis C viruses were negative. TTV DNA was amplified and detected using polymerase chain reaction followed by gel electrophoresis. RESULTS: Five of 99 (5%) samples obtained from donors with elevated ALT had TTV DNA detected by PCR, as compared to one of 146 (0.7%) of those with normal ALT (p = 0.006). Among those with elevated ALT, mean ALT values in patients with TTV (296 ± 305 U/L) were higher than in patients without TTV (95 ± 37 U/L), but the difference was not statistically significant (p = 0.08). The two samples with highest ALT values (both >450 U/L) were among the five samples with detectable TTV DNA in serum. CONCLUSIONS: Although TTV is not likely to explain the majority of elevated ALT cases in otherwise healthy blood donors, TTV infection may potentially be associated with some cases. Based on these findings, we propose that the role of TTV in the pathogenesis of acute and chronic liver diseases merits further investigation. (C) 2000 by Am. Coll. of Gastroenterology.

AB - OBJECTIVE: Recently a novel DNA virus (TT virus) has been identified in Japan and shown to be associated with elevated aminotransferase levels after blood transfusion. The exact role of TTV in the pathogenesis of liver disease is yet to be established. Our aim was to determine the prevalence and role of TTV in the pathogenesis of elevated transaminases in healthy blood donors in the absence of markers for viral hepatitis A-C. METHODS: Stored sera were collected from 99 healthy blood donors with elevated alanine amino transferase (ALT) values that were discovered at the time of blood donation. A total of 146 samples were obtained from healthy donors with normal ALT values who were used as controls. None of the patients or controls had a history of blood transfusion or had clinical signs of acute or chronic hepatitis. Serological markers for hepatitis A, hepatitis B, and hepatitis C viruses were negative. TTV DNA was amplified and detected using polymerase chain reaction followed by gel electrophoresis. RESULTS: Five of 99 (5%) samples obtained from donors with elevated ALT had TTV DNA detected by PCR, as compared to one of 146 (0.7%) of those with normal ALT (p = 0.006). Among those with elevated ALT, mean ALT values in patients with TTV (296 ± 305 U/L) were higher than in patients without TTV (95 ± 37 U/L), but the difference was not statistically significant (p = 0.08). The two samples with highest ALT values (both >450 U/L) were among the five samples with detectable TTV DNA in serum. CONCLUSIONS: Although TTV is not likely to explain the majority of elevated ALT cases in otherwise healthy blood donors, TTV infection may potentially be associated with some cases. Based on these findings, we propose that the role of TTV in the pathogenesis of acute and chronic liver diseases merits further investigation. (C) 2000 by Am. Coll. of Gastroenterology.

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