Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits

A. Olivos-Meza; J. S. Fitzsimmons; M. E. Casper; Q. Chen; K. N. An; T. J. Ruesink; S. W. O'Driscoll; G. G. Reinholz

doi:10.1016/j.joca.2010.06.003

Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits

A. Olivos-Meza, J. S. Fitzsimmons, M. E. Casper, Q. Chen, K. N. An, T. J. Ruesink, S. W. O'Driscoll, G. G. Reinholz

Orthopedic Surgery

Research output: Contribution to journal › Article › peer-review

20 Scopus citations

Abstract

Objective: To compare the efficacy of in situ transforming growth factor-beta1 (TGF-β1)-pretreated periosteum to untreated periosteum for regeneration of osteochondral tissue in rabbits. Methods: In the pretreatment group, 12 month-old New Zealand white rabbits received subperiosteal injections of 200. ng of TGF-β1 percutaneously in the medial side of the proximal tibia, 7 days prior to surgery. Control rabbits received no treatment prior surgery. Osteochondral transverse defects measuring 5. mm proximal to distal and spanning the entire width of the patellar groove were created and repaired with untreated or TGF-β1-pretreated periosteal grafts. Post-operatively the rabbits resumed normal cage activity for 6 weeks. Results: Complete filling of the defects with regenerated tissue was observed in both the TGF-β1-pretreated and control groups with reformation of the original contours of the patellar groove. The total histological score (modified O'Driscoll) in the TGF-β1-pretreated group, 20 (95% Confidence Interval (CI), 19-21), was significantly higher (P=0.0001) than the control group, 18 (16-19). The most notable improvements were in structural integrity and subchondral bone regeneration. No significant differences in glycosaminoglycan or type II collagen content, or equilibrium modulus were found between the surgical groups. The cambium of the periosteum regenerated at the graft harvest site was significantly thicker (P=0.0065) in the TGF-β1-pretreated rabbits, 121. μm (94-149), compared to controls, 74μm (52-96), after 6 weeks. Conclusions: This study demonstrates that in situ pretreatment of periosteum with TGF-β1 improves osteochondral tissue regeneration at 6-weeks post-op compared to untreated periosteum in 12 month-old rabbits.

Original language	English (US)
Pages (from-to)	1183-1191
Number of pages	9
Journal	Osteoarthritis and Cartilage
Volume	18
Issue number	9
DOIs	https://doi.org/10.1016/j.joca.2010.06.003
State	Published - Sep 2010

Keywords

Bone
Cartilage
Chondrogenesis
Joints
Periosteum
Regenerative medicine
Tissue engineering
Transforming growth factor-beta

ASJC Scopus subject areas

Rheumatology
Biomedical Engineering
Orthopedics and Sports Medicine

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10.1016/j.joca.2010.06.003

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Olivos-Meza, A., Fitzsimmons, J. S., Casper, M. E., Chen, Q., An, K. N., Ruesink, T. J., O'Driscoll, S. W., & Reinholz, G. G. (2010). Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits. Osteoarthritis and Cartilage, 18(9), 1183-1191. https://doi.org/10.1016/j.joca.2010.06.003

Olivos-Meza, A, Fitzsimmons, JS, Casper, ME, Chen, Q, An, KN, Ruesink, TJ, O'Driscoll, SW & Reinholz, GG 2010, 'Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits', Osteoarthritis and Cartilage, vol. 18, no. 9, pp. 1183-1191. https://doi.org/10.1016/j.joca.2010.06.003

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title = "Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits",

abstract = "Objective: To compare the efficacy of in situ transforming growth factor-beta1 (TGF-β1)-pretreated periosteum to untreated periosteum for regeneration of osteochondral tissue in rabbits. Methods: In the pretreatment group, 12 month-old New Zealand white rabbits received subperiosteal injections of 200. ng of TGF-β1 percutaneously in the medial side of the proximal tibia, 7 days prior to surgery. Control rabbits received no treatment prior surgery. Osteochondral transverse defects measuring 5. mm proximal to distal and spanning the entire width of the patellar groove were created and repaired with untreated or TGF-β1-pretreated periosteal grafts. Post-operatively the rabbits resumed normal cage activity for 6 weeks. Results: Complete filling of the defects with regenerated tissue was observed in both the TGF-β1-pretreated and control groups with reformation of the original contours of the patellar groove. The total histological score (modified O'Driscoll) in the TGF-β1-pretreated group, 20 (95% Confidence Interval (CI), 19-21), was significantly higher (P=0.0001) than the control group, 18 (16-19). The most notable improvements were in structural integrity and subchondral bone regeneration. No significant differences in glycosaminoglycan or type II collagen content, or equilibrium modulus were found between the surgical groups. The cambium of the periosteum regenerated at the graft harvest site was significantly thicker (P=0.0065) in the TGF-β1-pretreated rabbits, 121. μm (94-149), compared to controls, 74μm (52-96), after 6 weeks. Conclusions: This study demonstrates that in situ pretreatment of periosteum with TGF-β1 improves osteochondral tissue regeneration at 6-weeks post-op compared to untreated periosteum in 12 month-old rabbits.",

keywords = "Bone, Cartilage, Chondrogenesis, Joints, Periosteum, Regenerative medicine, Tissue engineering, Transforming growth factor-beta",

author = "A. Olivos-Meza and Fitzsimmons, {J. S.} and Casper, {M. E.} and Q. Chen and An, {K. N.} and Ruesink, {T. J.} and O'Driscoll, {S. W.} and Reinholz, {G. G.}",

note = "Funding Information: Role of the funding source: Funding for this project was provided by National Institutes of Health (NIH) and National Institute of Arthritis and Musculoskeletal Diseases (NIAMS) through grant AR52115 and supplements to this grant for M.E. Casper. Dr Olivos-Meza also received support from the Government of Veracruz State, and the University of Veracruz, Mexico. Mayo Clinic and the Department of Orthopedic Surgery provided additional funding support for this project. These funding sources were not involved in any other aspect of the study.",

year = "2010",

month = sep,

doi = "10.1016/j.joca.2010.06.003",

language = "English (US)",

volume = "18",

pages = "1183--1191",

journal = "Osteoarthritis and Cartilage",

issn = "1063-4584",

publisher = "W.B. Saunders Ltd",

number = "9",

}

TY - JOUR

T1 - Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits

AU - Olivos-Meza, A.

AU - Fitzsimmons, J. S.

AU - Casper, M. E.

AU - Chen, Q.

AU - An, K. N.

AU - Ruesink, T. J.

AU - O'Driscoll, S. W.

AU - Reinholz, G. G.

N1 - Funding Information: Role of the funding source: Funding for this project was provided by National Institutes of Health (NIH) and National Institute of Arthritis and Musculoskeletal Diseases (NIAMS) through grant AR52115 and supplements to this grant for M.E. Casper. Dr Olivos-Meza also received support from the Government of Veracruz State, and the University of Veracruz, Mexico. Mayo Clinic and the Department of Orthopedic Surgery provided additional funding support for this project. These funding sources were not involved in any other aspect of the study.

PY - 2010/9

Y1 - 2010/9

N2 - Objective: To compare the efficacy of in situ transforming growth factor-beta1 (TGF-β1)-pretreated periosteum to untreated periosteum for regeneration of osteochondral tissue in rabbits. Methods: In the pretreatment group, 12 month-old New Zealand white rabbits received subperiosteal injections of 200. ng of TGF-β1 percutaneously in the medial side of the proximal tibia, 7 days prior to surgery. Control rabbits received no treatment prior surgery. Osteochondral transverse defects measuring 5. mm proximal to distal and spanning the entire width of the patellar groove were created and repaired with untreated or TGF-β1-pretreated periosteal grafts. Post-operatively the rabbits resumed normal cage activity for 6 weeks. Results: Complete filling of the defects with regenerated tissue was observed in both the TGF-β1-pretreated and control groups with reformation of the original contours of the patellar groove. The total histological score (modified O'Driscoll) in the TGF-β1-pretreated group, 20 (95% Confidence Interval (CI), 19-21), was significantly higher (P=0.0001) than the control group, 18 (16-19). The most notable improvements were in structural integrity and subchondral bone regeneration. No significant differences in glycosaminoglycan or type II collagen content, or equilibrium modulus were found between the surgical groups. The cambium of the periosteum regenerated at the graft harvest site was significantly thicker (P=0.0065) in the TGF-β1-pretreated rabbits, 121. μm (94-149), compared to controls, 74μm (52-96), after 6 weeks. Conclusions: This study demonstrates that in situ pretreatment of periosteum with TGF-β1 improves osteochondral tissue regeneration at 6-weeks post-op compared to untreated periosteum in 12 month-old rabbits.

AB - Objective: To compare the efficacy of in situ transforming growth factor-beta1 (TGF-β1)-pretreated periosteum to untreated periosteum for regeneration of osteochondral tissue in rabbits. Methods: In the pretreatment group, 12 month-old New Zealand white rabbits received subperiosteal injections of 200. ng of TGF-β1 percutaneously in the medial side of the proximal tibia, 7 days prior to surgery. Control rabbits received no treatment prior surgery. Osteochondral transverse defects measuring 5. mm proximal to distal and spanning the entire width of the patellar groove were created and repaired with untreated or TGF-β1-pretreated periosteal grafts. Post-operatively the rabbits resumed normal cage activity for 6 weeks. Results: Complete filling of the defects with regenerated tissue was observed in both the TGF-β1-pretreated and control groups with reformation of the original contours of the patellar groove. The total histological score (modified O'Driscoll) in the TGF-β1-pretreated group, 20 (95% Confidence Interval (CI), 19-21), was significantly higher (P=0.0001) than the control group, 18 (16-19). The most notable improvements were in structural integrity and subchondral bone regeneration. No significant differences in glycosaminoglycan or type II collagen content, or equilibrium modulus were found between the surgical groups. The cambium of the periosteum regenerated at the graft harvest site was significantly thicker (P=0.0065) in the TGF-β1-pretreated rabbits, 121. μm (94-149), compared to controls, 74μm (52-96), after 6 weeks. Conclusions: This study demonstrates that in situ pretreatment of periosteum with TGF-β1 improves osteochondral tissue regeneration at 6-weeks post-op compared to untreated periosteum in 12 month-old rabbits.

KW - Bone

KW - Cartilage

KW - Chondrogenesis

KW - Joints

KW - Periosteum

KW - Regenerative medicine

KW - Tissue engineering

KW - Transforming growth factor-beta

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C2 - 20633683

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SN - 1063-4584

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EP - 1191

JO - Osteoarthritis and Cartilage

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ER -

Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits

Abstract

Keywords

ASJC Scopus subject areas

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