Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits

A. Olivos-Meza, J. S. Fitzsimmons, M. E. Casper, Q. Chen, K. N. An, T. J. Ruesink, S. W. O'Driscoll, G. G. Reinholz

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Objective: To compare the efficacy of in situ transforming growth factor-beta1 (TGF-β1)-pretreated periosteum to untreated periosteum for regeneration of osteochondral tissue in rabbits. Methods: In the pretreatment group, 12 month-old New Zealand white rabbits received subperiosteal injections of 200. ng of TGF-β1 percutaneously in the medial side of the proximal tibia, 7 days prior to surgery. Control rabbits received no treatment prior surgery. Osteochondral transverse defects measuring 5. mm proximal to distal and spanning the entire width of the patellar groove were created and repaired with untreated or TGF-β1-pretreated periosteal grafts. Post-operatively the rabbits resumed normal cage activity for 6 weeks. Results: Complete filling of the defects with regenerated tissue was observed in both the TGF-β1-pretreated and control groups with reformation of the original contours of the patellar groove. The total histological score (modified O'Driscoll) in the TGF-β1-pretreated group, 20 (95% Confidence Interval (CI), 19-21), was significantly higher (P=0.0001) than the control group, 18 (16-19). The most notable improvements were in structural integrity and subchondral bone regeneration. No significant differences in glycosaminoglycan or type II collagen content, or equilibrium modulus were found between the surgical groups. The cambium of the periosteum regenerated at the graft harvest site was significantly thicker (P=0.0065) in the TGF-β1-pretreated rabbits, 121. μm (94-149), compared to controls, 74μm (52-96), after 6 weeks. Conclusions: This study demonstrates that in situ pretreatment of periosteum with TGF-β1 improves osteochondral tissue regeneration at 6-weeks post-op compared to untreated periosteum in 12 month-old rabbits.

Original languageEnglish (US)
Pages (from-to)1183-1191
Number of pages9
JournalOsteoarthritis and Cartilage
Volume18
Issue number9
DOIs
StatePublished - Sep 2010

Fingerprint

Transforming Growth Factor beta1
Periosteum
Grafts
Tissue
Rabbits
Transplants
Surgery
Regeneration
Cambium
Tissue regeneration
Control Groups
Defects
Bone Regeneration
Collagen Type II
Structural integrity
Intercellular Signaling Peptides and Proteins
Glycosaminoglycans
Tibia
Collagen
Bone

Keywords

  • Bone
  • Cartilage
  • Chondrogenesis
  • Joints
  • Periosteum
  • Regenerative medicine
  • Tissue engineering
  • Transforming growth factor-beta

ASJC Scopus subject areas

  • Biomedical Engineering
  • Orthopedics and Sports Medicine
  • Rheumatology

Cite this

Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits. / Olivos-Meza, A.; Fitzsimmons, J. S.; Casper, M. E.; Chen, Q.; An, K. N.; Ruesink, T. J.; O'Driscoll, S. W.; Reinholz, G. G.

In: Osteoarthritis and Cartilage, Vol. 18, No. 9, 09.2010, p. 1183-1191.

Research output: Contribution to journalArticle

Olivos-Meza, A, Fitzsimmons, JS, Casper, ME, Chen, Q, An, KN, Ruesink, TJ, O'Driscoll, SW & Reinholz, GG 2010, 'Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits', Osteoarthritis and Cartilage, vol. 18, no. 9, pp. 1183-1191. https://doi.org/10.1016/j.joca.2010.06.003
Olivos-Meza, A. ; Fitzsimmons, J. S. ; Casper, M. E. ; Chen, Q. ; An, K. N. ; Ruesink, T. J. ; O'Driscoll, S. W. ; Reinholz, G. G. / Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits. In: Osteoarthritis and Cartilage. 2010 ; Vol. 18, No. 9. pp. 1183-1191.
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abstract = "Objective: To compare the efficacy of in situ transforming growth factor-beta1 (TGF-β1)-pretreated periosteum to untreated periosteum for regeneration of osteochondral tissue in rabbits. Methods: In the pretreatment group, 12 month-old New Zealand white rabbits received subperiosteal injections of 200. ng of TGF-β1 percutaneously in the medial side of the proximal tibia, 7 days prior to surgery. Control rabbits received no treatment prior surgery. Osteochondral transverse defects measuring 5. mm proximal to distal and spanning the entire width of the patellar groove were created and repaired with untreated or TGF-β1-pretreated periosteal grafts. Post-operatively the rabbits resumed normal cage activity for 6 weeks. Results: Complete filling of the defects with regenerated tissue was observed in both the TGF-β1-pretreated and control groups with reformation of the original contours of the patellar groove. The total histological score (modified O'Driscoll) in the TGF-β1-pretreated group, 20 (95{\%} Confidence Interval (CI), 19-21), was significantly higher (P=0.0001) than the control group, 18 (16-19). The most notable improvements were in structural integrity and subchondral bone regeneration. No significant differences in glycosaminoglycan or type II collagen content, or equilibrium modulus were found between the surgical groups. The cambium of the periosteum regenerated at the graft harvest site was significantly thicker (P=0.0065) in the TGF-β1-pretreated rabbits, 121. μm (94-149), compared to controls, 74μm (52-96), after 6 weeks. Conclusions: This study demonstrates that in situ pretreatment of periosteum with TGF-β1 improves osteochondral tissue regeneration at 6-weeks post-op compared to untreated periosteum in 12 month-old rabbits.",
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T1 - Pretreatment of periosteum with TGF-β1 in situ enhances the quality of osteochondral tissue regenerated from transplanted periosteal grafts in adult rabbits

AU - Olivos-Meza, A.

AU - Fitzsimmons, J. S.

AU - Casper, M. E.

AU - Chen, Q.

AU - An, K. N.

AU - Ruesink, T. J.

AU - O'Driscoll, S. W.

AU - Reinholz, G. G.

PY - 2010/9

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N2 - Objective: To compare the efficacy of in situ transforming growth factor-beta1 (TGF-β1)-pretreated periosteum to untreated periosteum for regeneration of osteochondral tissue in rabbits. Methods: In the pretreatment group, 12 month-old New Zealand white rabbits received subperiosteal injections of 200. ng of TGF-β1 percutaneously in the medial side of the proximal tibia, 7 days prior to surgery. Control rabbits received no treatment prior surgery. Osteochondral transverse defects measuring 5. mm proximal to distal and spanning the entire width of the patellar groove were created and repaired with untreated or TGF-β1-pretreated periosteal grafts. Post-operatively the rabbits resumed normal cage activity for 6 weeks. Results: Complete filling of the defects with regenerated tissue was observed in both the TGF-β1-pretreated and control groups with reformation of the original contours of the patellar groove. The total histological score (modified O'Driscoll) in the TGF-β1-pretreated group, 20 (95% Confidence Interval (CI), 19-21), was significantly higher (P=0.0001) than the control group, 18 (16-19). The most notable improvements were in structural integrity and subchondral bone regeneration. No significant differences in glycosaminoglycan or type II collagen content, or equilibrium modulus were found between the surgical groups. The cambium of the periosteum regenerated at the graft harvest site was significantly thicker (P=0.0065) in the TGF-β1-pretreated rabbits, 121. μm (94-149), compared to controls, 74μm (52-96), after 6 weeks. Conclusions: This study demonstrates that in situ pretreatment of periosteum with TGF-β1 improves osteochondral tissue regeneration at 6-weeks post-op compared to untreated periosteum in 12 month-old rabbits.

AB - Objective: To compare the efficacy of in situ transforming growth factor-beta1 (TGF-β1)-pretreated periosteum to untreated periosteum for regeneration of osteochondral tissue in rabbits. Methods: In the pretreatment group, 12 month-old New Zealand white rabbits received subperiosteal injections of 200. ng of TGF-β1 percutaneously in the medial side of the proximal tibia, 7 days prior to surgery. Control rabbits received no treatment prior surgery. Osteochondral transverse defects measuring 5. mm proximal to distal and spanning the entire width of the patellar groove were created and repaired with untreated or TGF-β1-pretreated periosteal grafts. Post-operatively the rabbits resumed normal cage activity for 6 weeks. Results: Complete filling of the defects with regenerated tissue was observed in both the TGF-β1-pretreated and control groups with reformation of the original contours of the patellar groove. The total histological score (modified O'Driscoll) in the TGF-β1-pretreated group, 20 (95% Confidence Interval (CI), 19-21), was significantly higher (P=0.0001) than the control group, 18 (16-19). The most notable improvements were in structural integrity and subchondral bone regeneration. No significant differences in glycosaminoglycan or type II collagen content, or equilibrium modulus were found between the surgical groups. The cambium of the periosteum regenerated at the graft harvest site was significantly thicker (P=0.0065) in the TGF-β1-pretreated rabbits, 121. μm (94-149), compared to controls, 74μm (52-96), after 6 weeks. Conclusions: This study demonstrates that in situ pretreatment of periosteum with TGF-β1 improves osteochondral tissue regeneration at 6-weeks post-op compared to untreated periosteum in 12 month-old rabbits.

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KW - Cartilage

KW - Chondrogenesis

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KW - Regenerative medicine

KW - Tissue engineering

KW - Transforming growth factor-beta

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