Preserved vascular integrity and enhanced survival following neuropilin-1 inhibition in a mouse model of CD8 T cell-initiated CNS vascular permeability

Georgette L. Suidan, Jonathan W. Dickerson, Holly L. Johnson, Theresa W. Chan, Kevin D. Pavelko, Istvan Pirko, Kim B. Seroogy, Aaron J. Johnson

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Background: Altered permeability of the blood-brain barrier (BBB) is a feature of numerous neurological conditions including multiple sclerosis, cerebral malaria, viral hemorrhagic fevers and acute hemorrhagic leukoencephalitis. Our laboratory has developed a murine model of CD8 T cell-initiated central nervous system (CNS) vascular permeability in which vascular endothelial growth factor (VEGF) signaling plays a prominent role in BBB disruption.Findings: In this study, we addressed the hypothesis that in vivo blockade of VEGF signal transduction through administration of peptide (ATWLPPR) to inhibit neuropilin-1 (NRP-1) would have a therapeutic effect following induction of CD8 T cell-initiated BBB disruption. We report that inhibition of NRP-1, a co-receptor that enhances VEGFR2 (flk-1) receptor activation, decreases vascular permeability, brain hemorrhage, and mortality in this model of CD8 T cell-initiated BBB disruption. We also examine the expression pattern of VEGFR2 (flk-1) and VEGFR1 (flt-1) mRNA expression during a time course of this condition. We find that viral infection of the brain leads to increased expression of flk-1 mRNA. In addition, flk-1 and flt-1 expression levels decrease in the striatum and hippocampus in later time points following induction of CD8 T cell-mediated BBB disruption.Conclusion: This study demonstrates that NRP-1 is a potential therapeutic target in neuro-inflammatory diseases involving BBB disruption and brain hemorrhage. Additionally, the reduction in VEGF receptors subsequent to BBB disruption could be involved in compensatory negative feedback as an attempt to reduce vascular permeability.

Original languageEnglish (US)
Article number218
JournalJournal of Neuroinflammation
Volume9
DOIs
StatePublished - Sep 18 2012

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Neuropilin-1
Capillary Permeability
Blood-Brain Barrier
Blood Vessels
Central Nervous System
T-Lymphocytes
Intracranial Hemorrhages
Vascular Endothelial Growth Factor A
Acute Hemorrhagic Leukoencephalitis
Viral Hemorrhagic Fevers
Cerebral Malaria
Messenger RNA
Vascular Endothelial Growth Factor Receptor
Therapeutic Uses
Virus Diseases
Multiple Sclerosis
Permeability
Signal Transduction
Hippocampus
Mortality

Keywords

  • Blood-brain barrier (BBB)
  • CD8 T cell
  • CNS vascular permeability
  • Fetal liver kinase 1 (flk-1)
  • FMS-related tyrosine kinase-1 (flt-1)
  • Neuropilin-1 (NRP-1)
  • Vascular endothelial growth factor (VEGF)

ASJC Scopus subject areas

  • Cellular and Molecular Neuroscience
  • Neurology
  • Immunology
  • Neuroscience(all)

Cite this

Preserved vascular integrity and enhanced survival following neuropilin-1 inhibition in a mouse model of CD8 T cell-initiated CNS vascular permeability. / Suidan, Georgette L.; Dickerson, Jonathan W.; Johnson, Holly L.; Chan, Theresa W.; Pavelko, Kevin D.; Pirko, Istvan; Seroogy, Kim B.; Johnson, Aaron J.

In: Journal of Neuroinflammation, Vol. 9, 218, 18.09.2012.

Research output: Contribution to journalArticle

Suidan, Georgette L. ; Dickerson, Jonathan W. ; Johnson, Holly L. ; Chan, Theresa W. ; Pavelko, Kevin D. ; Pirko, Istvan ; Seroogy, Kim B. ; Johnson, Aaron J. / Preserved vascular integrity and enhanced survival following neuropilin-1 inhibition in a mouse model of CD8 T cell-initiated CNS vascular permeability. In: Journal of Neuroinflammation. 2012 ; Vol. 9.
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AU - Suidan, Georgette L.

AU - Dickerson, Jonathan W.

AU - Johnson, Holly L.

AU - Chan, Theresa W.

AU - Pavelko, Kevin D.

AU - Pirko, Istvan

AU - Seroogy, Kim B.

AU - Johnson, Aaron J.

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