Presence of estrogen receptor β in urocortin 1-neurons in the mouse non-preganglionic Edinger-Westphal nucleus

Nicole M. Derks, Eric W. Roubos, Tamas Kozicz

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Adaptation to stress involves the activity of the hypothalamic-pituitary-adrenal (HPA-) axis. Urocortin 1 (Ucn1) coordinates responses to stressors. An increasing body of evidence suggests that such responses are sexually dimorphic and in females depend on the phase of the estrous cycle. Previously, in the non-preganglionic Edinger-Westphal nucleus (npEW), moderate immunostaining of the estrogen receptor α (ERα) was demonstrated, whereas estrogen receptor β (ERβ) was found to be more abundant. We have aimed at confirming the presence and identifying the type of ER in Ucn1-containing neurons in the npEW in the mouse, and at assessing whether the degree of Ucn1 mRNA expression is gender-related. Using immunocytochemistry, we could not demonstrate ERα-immunoreactivity in the npEW, but we did show a high density of ERβ-immunopositive neurons in the npEW of both male and female mice. A majority of Ucn1-positive neurons showed ERβ-immunoreactivity in their nuclei. In situ hybridization and RT-PCR did not reveal significant differences in both the number of neurons expressing Ucn1 mRNA and the strength of their Ucn1 mRNA expression. We will extend our gender comparison to other phases of the estrous cycle.

Original languageEnglish (US)
Pages (from-to)228-234
Number of pages7
JournalGeneral and Comparative Endocrinology
Volume153
Issue number1-3
DOIs
StatePublished - Jan 1 2007
Externally publishedYes

Fingerprint

Urocortins
Estrogen Receptors
neurons
Neurons
mice
Estrous Cycle
estrous cycle
Messenger RNA
gender
immunocytochemistry
in situ hybridization
In Situ Hybridization
Edinger-Westphal Nucleus
estrogen receptors
Immunohistochemistry
reverse transcriptase polymerase chain reaction
Polymerase Chain Reaction

Keywords

  • Estrogen receptor α
  • Estrogen receptor β
  • Non-preganglionic Edinger-Westphal nucleus
  • Urocortin 1

ASJC Scopus subject areas

  • Endocrinology

Cite this

Presence of estrogen receptor β in urocortin 1-neurons in the mouse non-preganglionic Edinger-Westphal nucleus. / Derks, Nicole M.; Roubos, Eric W.; Kozicz, Tamas.

In: General and Comparative Endocrinology, Vol. 153, No. 1-3, 01.01.2007, p. 228-234.

Research output: Contribution to journalArticle

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