Presence and secretion of atrial natriuretic peptide from cultured human aortic endothelial cells

R. R. Brandt, D. M. Heublein, M. T. Mattingly, M. R. Pittelkow, J. C. Burnett

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

The endothelium is the production site of several potent vasoactive substances that modulate vascular tone and growth. The present study was undertaken to investigate the presence and secretion of atrial natriuretic peptide (ANP) immunoreactivity from vascular endothelial cells. ANP immunoreactivity was present in cultured human aortic endothelial cells by both immunohistochemical staining and radioimmunoassay. ANP immunoreactivity was also detectable in culture medium from human aortic endothelial cells in low picogram concentrations. These findings suggest that vascular endothelium is a site of ANP production and secretion of ANP. There was a differential distribution of ANP and endothelin-1 (ET-1), witch a higher ANP concentration in cell extracts and a higher ET-1 concentration in cell culture media. Although ANP has been conceived as a circulating endocrine hormone, these findings are consistent with ANP functioning also as an autocrine and paracrine modulator in the regulation of vascular tone and growth.

Original languageEnglish (US)
Pages (from-to)H921-H925
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume268
Issue number2 37-2
DOIs
StatePublished - 1995

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

Fingerprint Dive into the research topics of 'Presence and secretion of atrial natriuretic peptide from cultured human aortic endothelial cells'. Together they form a unique fingerprint.

  • Cite this