Preparation of single cell suspensions enriched in mouse brain vascular cells for single-cell RNA sequencing

Akari Yamazaki, Francis Shue, Yu Yamazaki, Yuka A. Martens, Guojun Bu, Chia Chen Liu

Research output: Contribution to journalArticlepeer-review

Abstract

Cerebral blood vessels supply oxygen and nutrients, remove metabolic waste, and play a critical role in maintaining brain homeostasis. Cerebrovasculature is composed of heterogeneous populations of brain vascular cells (BVCs). A major challenge in effective cerebrovascular transcriptional profiling is high-quality BVC procurement, permitting high sequencing depth. Here, we establish cell isolation procedures for glio-vascular cell-enriched single-cell RNA sequencing enabling unbiased characterization of BVC transcriptional heterogeneity. Our approach can be used to address vascular-specific contribution to brain diseases. For complete details on the use and execution of this protocol, please refer to Yamazaki et al. (2021).

Original languageEnglish (US)
Article number100715
JournalSTAR Protocols
Volume2
Issue number3
DOIs
StatePublished - Sep 17 2021

Keywords

  • Cell isolation
  • Neuroscience
  • RNAseq
  • Single Cell

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Neuroscience(all)
  • Immunology and Microbiology(all)

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