Premature onset of fast axonal transport in bromophenylacetylurea neuropathy

an electrophoretic analysis of proteins exported into motor nerve

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6 Citations (Scopus)

Abstract

To test whether abnormal processing of proteins for fast axonal transport is involved in the neuropathy induced by BPAU (p-bromophenylacetylurea) we examined transport onset. [35S]Methionine was injected into the lumbar ventral horn of rats 2 weeks after BPAU, 400 mg/kg (i.p.) or vehicle. At intervals of 30-90 min consecutive 3-mm segments of the L4 and L5 ventral roots were digested for polyacrylamide gel electrophoresis and fluorography. Fast transported proteins were identified by comparison with samples from mid-thigh sciatic nerve ligated for 16 h after radiolabeling. A prominent 26 kDa band represented the earliest exported protein. It was usually absent at 30 min, but it entered the roots by 45 min. This band was consistently displaced further in BPAU nerve (n = 11) than in controls (n = 11). The mean difference was 5 ± 0.6 mm (P < 0.001). However, there was no difference in the apparent velocity of transport. These results imply premature onset of transport in BPAU neuropathy.

Original languageEnglish (US)
Pages (from-to)107-110
Number of pages4
JournalBrain Research
Volume509
Issue number1
DOIs
StatePublished - Feb 12 1990

Fingerprint

Axonal Transport
Photofluorography
Proteins
Spinal Nerve Roots
Sciatic Nerve
Horns
Thigh
Methionine
Polyacrylamide Gel Electrophoresis
4-bromophenylacetylurea

Keywords

  • Axonal transport
  • Experimental motor neuropathy
  • Gel electrophoresis
  • p-Bromophenylacetylurea
  • Radiolabeled protein
  • Toxin-induced peripheral neuropathy

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

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title = "Premature onset of fast axonal transport in bromophenylacetylurea neuropathy: an electrophoretic analysis of proteins exported into motor nerve",
abstract = "To test whether abnormal processing of proteins for fast axonal transport is involved in the neuropathy induced by BPAU (p-bromophenylacetylurea) we examined transport onset. [35S]Methionine was injected into the lumbar ventral horn of rats 2 weeks after BPAU, 400 mg/kg (i.p.) or vehicle. At intervals of 30-90 min consecutive 3-mm segments of the L4 and L5 ventral roots were digested for polyacrylamide gel electrophoresis and fluorography. Fast transported proteins were identified by comparison with samples from mid-thigh sciatic nerve ligated for 16 h after radiolabeling. A prominent 26 kDa band represented the earliest exported protein. It was usually absent at 30 min, but it entered the roots by 45 min. This band was consistently displaced further in BPAU nerve (n = 11) than in controls (n = 11). The mean difference was 5 ± 0.6 mm (P < 0.001). However, there was no difference in the apparent velocity of transport. These results imply premature onset of transport in BPAU neuropathy.",
keywords = "Axonal transport, Experimental motor neuropathy, Gel electrophoresis, p-Bromophenylacetylurea, Radiolabeled protein, Toxin-induced peripheral neuropathy",
author = "Nobuyuki Oka and Brimijoin, {William Stephen}",
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AU - Brimijoin, William Stephen

PY - 1990/2/12

Y1 - 1990/2/12

N2 - To test whether abnormal processing of proteins for fast axonal transport is involved in the neuropathy induced by BPAU (p-bromophenylacetylurea) we examined transport onset. [35S]Methionine was injected into the lumbar ventral horn of rats 2 weeks after BPAU, 400 mg/kg (i.p.) or vehicle. At intervals of 30-90 min consecutive 3-mm segments of the L4 and L5 ventral roots were digested for polyacrylamide gel electrophoresis and fluorography. Fast transported proteins were identified by comparison with samples from mid-thigh sciatic nerve ligated for 16 h after radiolabeling. A prominent 26 kDa band represented the earliest exported protein. It was usually absent at 30 min, but it entered the roots by 45 min. This band was consistently displaced further in BPAU nerve (n = 11) than in controls (n = 11). The mean difference was 5 ± 0.6 mm (P < 0.001). However, there was no difference in the apparent velocity of transport. These results imply premature onset of transport in BPAU neuropathy.

AB - To test whether abnormal processing of proteins for fast axonal transport is involved in the neuropathy induced by BPAU (p-bromophenylacetylurea) we examined transport onset. [35S]Methionine was injected into the lumbar ventral horn of rats 2 weeks after BPAU, 400 mg/kg (i.p.) or vehicle. At intervals of 30-90 min consecutive 3-mm segments of the L4 and L5 ventral roots were digested for polyacrylamide gel electrophoresis and fluorography. Fast transported proteins were identified by comparison with samples from mid-thigh sciatic nerve ligated for 16 h after radiolabeling. A prominent 26 kDa band represented the earliest exported protein. It was usually absent at 30 min, but it entered the roots by 45 min. This band was consistently displaced further in BPAU nerve (n = 11) than in controls (n = 11). The mean difference was 5 ± 0.6 mm (P < 0.001). However, there was no difference in the apparent velocity of transport. These results imply premature onset of transport in BPAU neuropathy.

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KW - Toxin-induced peripheral neuropathy

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