Pregnancy history and blood-borne microvesicles in middle aged women with and without coronary artery calcification

Virginia M Miller, Vesna D Garovic, Kent R Bailey, Brian D. Lahr, Michelle M Mielke, Wendy M. White, Muthuvel Jayachandran

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Background and aims Having a history of preeclampsia increases the risk for future coronary artery calcification (CAC). This study evaluated the association of blood-borne, cell-derived microvesicles (MV) with CAC in middle-aged women. Methods Twelve pre-selected, antigen-specific MV were measured by digital flow cytometry in the blood of age- and parity-matched women (median age 60 years) without a history of cardiovascular events, but with either a history of preeclampsia (PE, n = 39) or normotensive pregnancy (NP, n = 40). CAC was determined by computed tomography. Results CAC scores ranged from 0 to 47 and 0–602 Agatston Units in the NP and PE groups, respectively. Waist circumference and insulin resistance were greatest in PE women with CAC. MV positive for tissue factor or stem/progenitor cell antigen (CD117) differed between NP and PE groups. In univariate analysis, those positive for tissue factor, ICAM-1, stem cells, and adipocytes (P16-set) antigens associated with CAC in the PE group. Principal components (PC) analysis reduced the MV variables to three independent dimensions. PC1 showed a modest correlation with CAC scores in the PE group (ρ = 0.31, p = 0.06) and associated with CAC in a multivariable model on pooled groups that included all 3 PC variables when adjusted for pregnancy status (p = 0.03). The association was lost when corrected for body mass index or waist circumference. Conclusions In women with a history of PE and elevated metabolic risk profile, a group of specific antigen-positive MV associated with CAC. These MV may reflect cellular processes associated with CAC. Their diagnostic potential for CAC remains to be determined.

Original languageEnglish (US)
Pages (from-to)150-155
Number of pages6
JournalAtherosclerosis
Volume253
DOIs
StatePublished - Oct 1 2016

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Reproductive History
Coronary Vessels
Stem Cells
Waist Circumference
Thromboplastin
Pre-Eclampsia
Antigens
Proto-Oncogene Proteins c-kit
Pregnancy
Metabolome
Intercellular Adhesion Molecule-1
Parity
Principal Component Analysis
Adipocytes
Insulin Resistance
Blood Cells
Flow Cytometry
Body Mass Index
Tomography

Keywords

  • Extracellular vesicles
  • Glucose
  • Hypertension
  • Insulin
  • Microparticles

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

Cite this

Pregnancy history and blood-borne microvesicles in middle aged women with and without coronary artery calcification. / Miller, Virginia M; Garovic, Vesna D; Bailey, Kent R; Lahr, Brian D.; Mielke, Michelle M; White, Wendy M.; Jayachandran, Muthuvel.

In: Atherosclerosis, Vol. 253, 01.10.2016, p. 150-155.

Research output: Contribution to journalArticle

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