Abstract
Background and aims Having a history of preeclampsia increases the risk for future coronary artery calcification (CAC). This study evaluated the association of blood-borne, cell-derived microvesicles (MV) with CAC in middle-aged women. Methods Twelve pre-selected, antigen-specific MV were measured by digital flow cytometry in the blood of age- and parity-matched women (median age 60 years) without a history of cardiovascular events, but with either a history of preeclampsia (PE, n = 39) or normotensive pregnancy (NP, n = 40). CAC was determined by computed tomography. Results CAC scores ranged from 0 to 47 and 0–602 Agatston Units in the NP and PE groups, respectively. Waist circumference and insulin resistance were greatest in PE women with CAC. MV positive for tissue factor or stem/progenitor cell antigen (CD117) differed between NP and PE groups. In univariate analysis, those positive for tissue factor, ICAM-1, stem cells, and adipocytes (P16-set) antigens associated with CAC in the PE group. Principal components (PC) analysis reduced the MV variables to three independent dimensions. PC1 showed a modest correlation with CAC scores in the PE group (ρ = 0.31, p = 0.06) and associated with CAC in a multivariable model on pooled groups that included all 3 PC variables when adjusted for pregnancy status (p = 0.03). The association was lost when corrected for body mass index or waist circumference. Conclusions In women with a history of PE and elevated metabolic risk profile, a group of specific antigen-positive MV associated with CAC. These MV may reflect cellular processes associated with CAC. Their diagnostic potential for CAC remains to be determined.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 150-155 |
| Number of pages | 6 |
| Journal | Atherosclerosis |
| Volume | 253 |
| DOIs | |
| State | Published - Oct 1 2016 |
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Keywords
- Extracellular vesicles
- Glucose
- Hypertension
- Insulin
- Microparticles
ASJC Scopus subject areas
- Cardiology and Cardiovascular Medicine
Cite this
Pregnancy history and blood-borne microvesicles in middle aged women with and without coronary artery calcification. / Miller, Virginia M; Garovic, Vesna D; Bailey, Kent R; Lahr, Brian D.; Mielke, Michelle M; White, Wendy M.; Jayachandran, Muthuvel.
In: Atherosclerosis, Vol. 253, 01.10.2016, p. 150-155.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Pregnancy history and blood-borne microvesicles in middle aged women with and without coronary artery calcification
AU - Miller, Virginia M
AU - Garovic, Vesna D
AU - Bailey, Kent R
AU - Lahr, Brian D.
AU - Mielke, Michelle M
AU - White, Wendy M.
AU - Jayachandran, Muthuvel
PY - 2016/10/1
Y1 - 2016/10/1
N2 - Background and aims Having a history of preeclampsia increases the risk for future coronary artery calcification (CAC). This study evaluated the association of blood-borne, cell-derived microvesicles (MV) with CAC in middle-aged women. Methods Twelve pre-selected, antigen-specific MV were measured by digital flow cytometry in the blood of age- and parity-matched women (median age 60 years) without a history of cardiovascular events, but with either a history of preeclampsia (PE, n = 39) or normotensive pregnancy (NP, n = 40). CAC was determined by computed tomography. Results CAC scores ranged from 0 to 47 and 0–602 Agatston Units in the NP and PE groups, respectively. Waist circumference and insulin resistance were greatest in PE women with CAC. MV positive for tissue factor or stem/progenitor cell antigen (CD117) differed between NP and PE groups. In univariate analysis, those positive for tissue factor, ICAM-1, stem cells, and adipocytes (P16-set) antigens associated with CAC in the PE group. Principal components (PC) analysis reduced the MV variables to three independent dimensions. PC1 showed a modest correlation with CAC scores in the PE group (ρ = 0.31, p = 0.06) and associated with CAC in a multivariable model on pooled groups that included all 3 PC variables when adjusted for pregnancy status (p = 0.03). The association was lost when corrected for body mass index or waist circumference. Conclusions In women with a history of PE and elevated metabolic risk profile, a group of specific antigen-positive MV associated with CAC. These MV may reflect cellular processes associated with CAC. Their diagnostic potential for CAC remains to be determined.
AB - Background and aims Having a history of preeclampsia increases the risk for future coronary artery calcification (CAC). This study evaluated the association of blood-borne, cell-derived microvesicles (MV) with CAC in middle-aged women. Methods Twelve pre-selected, antigen-specific MV were measured by digital flow cytometry in the blood of age- and parity-matched women (median age 60 years) without a history of cardiovascular events, but with either a history of preeclampsia (PE, n = 39) or normotensive pregnancy (NP, n = 40). CAC was determined by computed tomography. Results CAC scores ranged from 0 to 47 and 0–602 Agatston Units in the NP and PE groups, respectively. Waist circumference and insulin resistance were greatest in PE women with CAC. MV positive for tissue factor or stem/progenitor cell antigen (CD117) differed between NP and PE groups. In univariate analysis, those positive for tissue factor, ICAM-1, stem cells, and adipocytes (P16-set) antigens associated with CAC in the PE group. Principal components (PC) analysis reduced the MV variables to three independent dimensions. PC1 showed a modest correlation with CAC scores in the PE group (ρ = 0.31, p = 0.06) and associated with CAC in a multivariable model on pooled groups that included all 3 PC variables when adjusted for pregnancy status (p = 0.03). The association was lost when corrected for body mass index or waist circumference. Conclusions In women with a history of PE and elevated metabolic risk profile, a group of specific antigen-positive MV associated with CAC. These MV may reflect cellular processes associated with CAC. Their diagnostic potential for CAC remains to be determined.
KW - Extracellular vesicles
KW - Glucose
KW - Hypertension
KW - Insulin
KW - Microparticles
UR - http://www.scopus.com/inward/record.url?scp=84988038754&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84988038754&partnerID=8YFLogxK
U2 - 10.1016/j.atherosclerosis.2016.09.006
DO - 10.1016/j.atherosclerosis.2016.09.006
M3 - Article
C2 - 27639028
AN - SCOPUS:84988038754
VL - 253
SP - 150
EP - 155
JO - Atherosclerosis
JF - Atherosclerosis
SN - 0021-9150
ER -