Pregnancy-associated plasma protein-A (PAPP-A) in ovine, bovine, porcine, and equine ovarian follicles: Involvement in IGF binding protein-4 proteolytic degradation and mRNA expression during follicular development

Sabine Mazerbourg, Michael T. Overgaard, Claus Oxvig, Michael Christiansen, Cheryl A Conover, Ingrid Laurendeau, Michel Vidaud, Gwenola Tosser-Klopp, Jürgen Zapf, Philippe Monget

Research output: Contribution to journalArticle

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Abstract

IGF binding protein-4 (IGFBP-4) proteolytic degradation is a common feature of preovulatory follicles from human, ovine, bovine, porcine, and equine ovary. In all these species, the protease is a zinc-dependent metalloprotease and its ability to degrade IGFBP-4 is IGF dependent. The human intrafollicular IGFBP-4-degrading protease has recently been identified as pregnancy-associated plasma protein-A (PAPP-A). The aim of this study was to investigate whether PAPP-A is also involved in IGFBP-4 degradation in ovine, bovine, porcine, and equine preovulatory follicles and to study the expression of PAPP-A mRNA in bovine and porcine granulosa cells from different classes of follicles. Immunoneutralization and immunoprecipitation with polyclonal antibodies raised against human PAPP-A inhibited IGFBP-4 proteolytic degradation in preovulatory follicular fluid from the four species studied. As previously reported for the intrafollicular proteolytic activity degrading IGFBP-4, recombinant human PAPP-A generated in vitro 17- and 10-kDa IGFBP-4-proteolytic fragments. Recombinant PAPP-A activity was also shown to be IGF dependent and was inhibited by heparin-binding domain-containing peptides. In all mammalian species studied, the PAPP-A sequences showed high degree of identity. Moreover, the PAPP-A gene was localized on porcine chromosome 1 (1q29-1q213), in agreement with the localization of human PAPP-A gene on human chromosome 9q33.1. In bovine and porcine ovaries, real-time quantitative RT-PCR showed that PAPP-A mRNA expression in granulosa cells was maximal in fully differentiated follicles and was positively correlated with expression of P450 aromatase and LH receptor mRNAs. Overall, these data show that PAPP-A is responsible for IGFBP-4 degradation in ovine, bovine, porcine, and equine preovulatory follicles. The high expression of PAPP-A mRNA in granulosa cells from large, differentiated follicles suggest that it is a new functional marker of follicular development.

Original languageEnglish (US)
Pages (from-to)5243-5253
Number of pages11
JournalEndocrinology
Volume142
Issue number12
DOIs
StatePublished - 2001

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Insulin-Like Growth Factor Binding Protein 4
Pregnancy-Associated Plasma Protein-A
Ovarian Follicle
RNA Stability
Horses
Sheep
Swine
Granulosa Cells
Messenger RNA
Proteolysis
Ovary
Peptide Hydrolases
LH Receptors
Follicular Fluid
Aromatase
Chromosomes, Human, Pair 1
Human Chromosomes
Metalloproteases
Immunoprecipitation
Genes

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Pregnancy-associated plasma protein-A (PAPP-A) in ovine, bovine, porcine, and equine ovarian follicles : Involvement in IGF binding protein-4 proteolytic degradation and mRNA expression during follicular development. / Mazerbourg, Sabine; Overgaard, Michael T.; Oxvig, Claus; Christiansen, Michael; Conover, Cheryl A; Laurendeau, Ingrid; Vidaud, Michel; Tosser-Klopp, Gwenola; Zapf, Jürgen; Monget, Philippe.

In: Endocrinology, Vol. 142, No. 12, 2001, p. 5243-5253.

Research output: Contribution to journalArticle

Mazerbourg, Sabine ; Overgaard, Michael T. ; Oxvig, Claus ; Christiansen, Michael ; Conover, Cheryl A ; Laurendeau, Ingrid ; Vidaud, Michel ; Tosser-Klopp, Gwenola ; Zapf, Jürgen ; Monget, Philippe. / Pregnancy-associated plasma protein-A (PAPP-A) in ovine, bovine, porcine, and equine ovarian follicles : Involvement in IGF binding protein-4 proteolytic degradation and mRNA expression during follicular development. In: Endocrinology. 2001 ; Vol. 142, No. 12. pp. 5243-5253.
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abstract = "IGF binding protein-4 (IGFBP-4) proteolytic degradation is a common feature of preovulatory follicles from human, ovine, bovine, porcine, and equine ovary. In all these species, the protease is a zinc-dependent metalloprotease and its ability to degrade IGFBP-4 is IGF dependent. The human intrafollicular IGFBP-4-degrading protease has recently been identified as pregnancy-associated plasma protein-A (PAPP-A). The aim of this study was to investigate whether PAPP-A is also involved in IGFBP-4 degradation in ovine, bovine, porcine, and equine preovulatory follicles and to study the expression of PAPP-A mRNA in bovine and porcine granulosa cells from different classes of follicles. Immunoneutralization and immunoprecipitation with polyclonal antibodies raised against human PAPP-A inhibited IGFBP-4 proteolytic degradation in preovulatory follicular fluid from the four species studied. As previously reported for the intrafollicular proteolytic activity degrading IGFBP-4, recombinant human PAPP-A generated in vitro 17- and 10-kDa IGFBP-4-proteolytic fragments. Recombinant PAPP-A activity was also shown to be IGF dependent and was inhibited by heparin-binding domain-containing peptides. In all mammalian species studied, the PAPP-A sequences showed high degree of identity. Moreover, the PAPP-A gene was localized on porcine chromosome 1 (1q29-1q213), in agreement with the localization of human PAPP-A gene on human chromosome 9q33.1. In bovine and porcine ovaries, real-time quantitative RT-PCR showed that PAPP-A mRNA expression in granulosa cells was maximal in fully differentiated follicles and was positively correlated with expression of P450 aromatase and LH receptor mRNAs. Overall, these data show that PAPP-A is responsible for IGFBP-4 degradation in ovine, bovine, porcine, and equine preovulatory follicles. The high expression of PAPP-A mRNA in granulosa cells from large, differentiated follicles suggest that it is a new functional marker of follicular development.",
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AU - Mazerbourg, Sabine

AU - Overgaard, Michael T.

AU - Oxvig, Claus

AU - Christiansen, Michael

AU - Conover, Cheryl A

AU - Laurendeau, Ingrid

AU - Vidaud, Michel

AU - Tosser-Klopp, Gwenola

AU - Zapf, Jürgen

AU - Monget, Philippe

PY - 2001

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