Prefoldin 1 promotes EMT and lung cancer progression by suppressing cyclin A expression

D. Wang, W. Shi, Y. Tang, Y. Liu, K. He, Y. Hu, J. Li, Yanan D Yang, J. Song

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

Prefoldin (PFDN) is a co-chaperone protein that is primarily known for its classic cytoplasmic functions in the folding of actin and tubulin monomers during cytoskeletal assembly. Here, we report a marked increase in prefoldin subunit 1 (PFDN1) levels during the transforming growth factor (TGF)-β1-mediated epithelial-mesenchymal transition (EMT) and in human lung tumor tissues. Interestingly, the nuclear localization of PFDN1 was also detected. These observations suggest that PFDN1 may be essential for important novel functions. Overexpression of PFDN1 induced EMT and cell invasion. In sharp contrast, knockdown of PFDN1 generated the opposite effects. Overexpression of PFDN1 was also found to induce lung tumor growth and metastasis. Further experiments showed that PFDN1 overexpression inhibits the expression of cyclin A. PFDN1 suppressed cyclin A expression by directly interacting with the cyclin A promoter at the transcriptional start site. Strikingly, cyclin A overexpression abolished the above PFDN1-mediated effects on the behavior of lung cancer cells, whereas cyclin A knockdown alone induced EMT and increased cell migration and invasion ability. This study reveals that the TGF-β1/PFDN1/cyclin A axis is essential for EMT induction and metastasis of lung cancer cells.Oncogene advance online publication, 3 October 2016; doi:10.1038/onc.2016.257.

Original languageEnglish (US)
JournalOncogene
DOIs
StateAccepted/In press - Oct 3 2016
Externally publishedYes

Fingerprint

Cyclin A
Epithelial-Mesenchymal Transition
Lung Neoplasms
Transforming Growth Factors
prefoldin
Neoplasm Metastasis
Lung
Aptitude
Tubulin
Oncogenes
Cell Movement
Publications
Actins
Neoplasms

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cancer Research

Cite this

Wang, D., Shi, W., Tang, Y., Liu, Y., He, K., Hu, Y., ... Song, J. (Accepted/In press). Prefoldin 1 promotes EMT and lung cancer progression by suppressing cyclin A expression. Oncogene. https://doi.org/10.1038/onc.2016.257

Prefoldin 1 promotes EMT and lung cancer progression by suppressing cyclin A expression. / Wang, D.; Shi, W.; Tang, Y.; Liu, Y.; He, K.; Hu, Y.; Li, J.; Yang, Yanan D; Song, J.

In: Oncogene, 03.10.2016.

Research output: Contribution to journalArticle

Wang, D. ; Shi, W. ; Tang, Y. ; Liu, Y. ; He, K. ; Hu, Y. ; Li, J. ; Yang, Yanan D ; Song, J. / Prefoldin 1 promotes EMT and lung cancer progression by suppressing cyclin A expression. In: Oncogene. 2016.
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