Preferential repair of the N-ras gene in K 562 cells after exposure to cisplatin

Wolfram Dempke, Wieland Voigt, Axel F Grothey, Hans Joachim Schmoll

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Cisplatin is one of the most active and widely used anticancer drugs; however, its clinical efficiacy is often limited by the development of resistance. Since several studies indicated that ras oncogenes may modulate the cellular response to cisplatin or radiation, we investigated the gene-specific repair of the N-ras gene in the human K 562 cell line after exposure to cisplatin using a novel nonradioactive polymerase chain reaction inhibition assay. This assay is based on the fact that DNA lesions can block the Taq polymerase and thereby result in a decreased amplification of a damaged segment compared to the amplification of the same segment in a non-damaged template. The overall genomic repair rate was measured by atomic absorption spectroscopy. Immediately after cisplatin exposure no amplification segment was observed. However, a complete restoration of the N-ras gene (2.4 kb) was seen after 8 h posttreatment incubation. In contrast, only 60% of the overall genome was repaired at this time. Our results clearly indicate that cisplatin-induced DNA lesions are more efficiently removed from transcribed regions within the DNA, suggesting that the efficiency of DNA repair in a given gene may be correlated to its transcriptional activity. Since ras oncogenes control several cellular signal transduction pathways, known to be involved in DNA damage response, preferential repair of the N-ras gene could therefore be an important step to prevent inactivation of cellular defense mechanisms after exposure to genotoxic agents.

Original languageEnglish (US)
Pages (from-to)545-549
Number of pages5
JournalAnti-Cancer Drugs
Volume10
Issue number6
StatePublished - 1999
Externally publishedYes

Fingerprint

ras Genes
Cisplatin
DNA
Taq Polymerase
DNA Repair
Genes
DNA Damage
Signal Transduction
Spectrum Analysis
Genome
Radiation
Cell Line
Polymerase Chain Reaction
Pharmaceutical Preparations

Keywords

  • Cisplatin
  • Gene specific repair
  • N-ras

ASJC Scopus subject areas

  • Cancer Research
  • Oncology
  • Pharmacology

Cite this

Dempke, W., Voigt, W., Grothey, A. F., & Schmoll, H. J. (1999). Preferential repair of the N-ras gene in K 562 cells after exposure to cisplatin. Anti-Cancer Drugs, 10(6), 545-549.

Preferential repair of the N-ras gene in K 562 cells after exposure to cisplatin. / Dempke, Wolfram; Voigt, Wieland; Grothey, Axel F; Schmoll, Hans Joachim.

In: Anti-Cancer Drugs, Vol. 10, No. 6, 1999, p. 545-549.

Research output: Contribution to journalArticle

Dempke, W, Voigt, W, Grothey, AF & Schmoll, HJ 1999, 'Preferential repair of the N-ras gene in K 562 cells after exposure to cisplatin', Anti-Cancer Drugs, vol. 10, no. 6, pp. 545-549.
Dempke W, Voigt W, Grothey AF, Schmoll HJ. Preferential repair of the N-ras gene in K 562 cells after exposure to cisplatin. Anti-Cancer Drugs. 1999;10(6):545-549.
Dempke, Wolfram ; Voigt, Wieland ; Grothey, Axel F ; Schmoll, Hans Joachim. / Preferential repair of the N-ras gene in K 562 cells after exposure to cisplatin. In: Anti-Cancer Drugs. 1999 ; Vol. 10, No. 6. pp. 545-549.
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