Preferential expression of the secreted and membrane forms of tumor endothelial marker 7 transcripts in osteosarcoma

Chandralekha Halder, Christian Ossendorf, Avudaiappan Maran, Michael Yaszemski, Mark E. Bolander, Bruno Fuchs, Gobinda Sarkar

Research output: Contribution to journalArticle

7 Scopus citations

Abstract

Background: High expression of tumor endothelial marker 7 (TEM7) is correlated with osteogenic sarcoma (OS) metastasis and poor survival of patients. The TEM7 gene produces four alternatively spliced transcripts with distinct functional domains; the expression pattern of these transcripts in OS is unknown. Materials and Methods: mRNA expression was assessed in 5 OS cell lines, 7 normal bone, and 9 OS tumor specimens by reverse transcriptase polymerase chain reaction. Results: All OS cell lines, 6/9 tumors but none of the bone specimens expressed mRNA of TEM7 secreted forms 1 and 2. A total of 3/5 OS cell lines, 8/9 of tumors and 4/7 of bone specimens expressed mRNA of the TEM7 intracellular form. One out of 5 cell lines, 2/7 tumors and none of the bone specimens expressed mRNA of the TEM7 membrane form. The secreted forms had 20-fold higher expression in metastatic (LM7) compared to non-metastatic (SAOS-2) cells. Conclusion: The mRNA of secreted and the membrane forms of TEM7 are preferentially expressed in OS.

Original languageEnglish (US)
Pages (from-to)4317-4322
Number of pages6
JournalAnticancer research
Volume29
Issue number11
StatePublished - Nov 1 2009

Keywords

  • Alternative splicing
  • Metastasis
  • Osteosarcoma
  • PCR
  • TEM7

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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    Halder, C., Ossendorf, C., Maran, A., Yaszemski, M., Bolander, M. E., Fuchs, B., & Sarkar, G. (2009). Preferential expression of the secreted and membrane forms of tumor endothelial marker 7 transcripts in osteosarcoma. Anticancer research, 29(11), 4317-4322.