Precision of CAPILLARYS 2 for the detection of hemoglobin variants based on their migration positions

Jean Riou; Jessica Szuberski; Christian Godart; Henri Wajcman; Jennifer L. Oliveira; James D. Hoyer; Josiane Bardakdjian-Michau

doi:10.1093/AJCP/AQX148

Precision of CAPILLARYS 2 for the detection of hemoglobin variants based on their migration positions

Jean Riou, Jessica Szuberski, Christian Godart, Henri Wajcman, Jennifer L. Oliveira, James D. Hoyer, Josiane Bardakdjian-Michau

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

6 Scopus citations

Abstract

Objectives: In this report, we evaluated utility of the capillary electrophoresis (CE) migration position of the CAPILLARYS 2 CE instrument. Methods: The precision of this x-axis number was determined on a selection of common hemoglobin (Hb) variants (Hb S, Hb C, Hb D-Punjab, Hb E, Hb Hope), and the reproducibility of this number was evaluated by comparing the results obtained by two large reference laboratories on 81 Hb variants. Additionally, the CE migration position is given for a total of 409 Hb variants. Results: The x-axis migration position showed excellent intra- and interassay precision. Comparison of Hb variants seen by both laboratories showed that 83% had a difference in migration position of 1 unit or less. Only three rare Hb variants showed a difference of more than 2 units. Conclusion: In summary, the CE migration position is a reproducible value and can be used as an aid in the identification of Hb variants.

Original language	English (US)
Pages (from-to)	172-180
Number of pages	9
Journal	American journal of clinical pathology
Volume	149
Issue number	2
DOIs	https://doi.org/10.1093/AJCP/AQX148
State	Published - Feb 1 2018

Keywords

CAPILLARYS 2
Capillary electrophoresis
Hemoglobinopathies

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1093/AJCP/AQX148

Cite this

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title = "Precision of CAPILLARYS 2 for the detection of hemoglobin variants based on their migration positions",

abstract = "Objectives: In this report, we evaluated utility of the capillary electrophoresis (CE) migration position of the CAPILLARYS 2 CE instrument. Methods: The precision of this x-axis number was determined on a selection of common hemoglobin (Hb) variants (Hb S, Hb C, Hb D-Punjab, Hb E, Hb Hope), and the reproducibility of this number was evaluated by comparing the results obtained by two large reference laboratories on 81 Hb variants. Additionally, the CE migration position is given for a total of 409 Hb variants. Results: The x-axis migration position showed excellent intra- and interassay precision. Comparison of Hb variants seen by both laboratories showed that 83% had a difference in migration position of 1 unit or less. Only three rare Hb variants showed a difference of more than 2 units. Conclusion: In summary, the CE migration position is a reproducible value and can be used as an aid in the identification of Hb variants.",

keywords = "CAPILLARYS 2, Capillary electrophoresis, Hemoglobinopathies",

author = "Jean Riou and Jessica Szuberski and Christian Godart and Henri Wajcman and Oliveira, {Jennifer L.} and Hoyer, {James D.} and Josiane Bardakdjian-Michau",

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doi = "10.1093/AJCP/AQX148",

language = "English (US)",

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journal = "American journal of clinical pathology",

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AU - Riou, Jean

AU - Szuberski, Jessica

AU - Godart, Christian

AU - Wajcman, Henri

AU - Oliveira, Jennifer L.

AU - Hoyer, James D.

AU - Bardakdjian-Michau, Josiane

PY - 2018/2/1

Y1 - 2018/2/1

N2 - Objectives: In this report, we evaluated utility of the capillary electrophoresis (CE) migration position of the CAPILLARYS 2 CE instrument. Methods: The precision of this x-axis number was determined on a selection of common hemoglobin (Hb) variants (Hb S, Hb C, Hb D-Punjab, Hb E, Hb Hope), and the reproducibility of this number was evaluated by comparing the results obtained by two large reference laboratories on 81 Hb variants. Additionally, the CE migration position is given for a total of 409 Hb variants. Results: The x-axis migration position showed excellent intra- and interassay precision. Comparison of Hb variants seen by both laboratories showed that 83% had a difference in migration position of 1 unit or less. Only three rare Hb variants showed a difference of more than 2 units. Conclusion: In summary, the CE migration position is a reproducible value and can be used as an aid in the identification of Hb variants.

AB - Objectives: In this report, we evaluated utility of the capillary electrophoresis (CE) migration position of the CAPILLARYS 2 CE instrument. Methods: The precision of this x-axis number was determined on a selection of common hemoglobin (Hb) variants (Hb S, Hb C, Hb D-Punjab, Hb E, Hb Hope), and the reproducibility of this number was evaluated by comparing the results obtained by two large reference laboratories on 81 Hb variants. Additionally, the CE migration position is given for a total of 409 Hb variants. Results: The x-axis migration position showed excellent intra- and interassay precision. Comparison of Hb variants seen by both laboratories showed that 83% had a difference in migration position of 1 unit or less. Only three rare Hb variants showed a difference of more than 2 units. Conclusion: In summary, the CE migration position is a reproducible value and can be used as an aid in the identification of Hb variants.

KW - CAPILLARYS 2

KW - Capillary electrophoresis

KW - Hemoglobinopathies

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Precision of CAPILLARYS 2 for the detection of hemoglobin variants based on their migration positions

Abstract

Keywords

ASJC Scopus subject areas

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