Precision of CAPILLARYS 2 for the detection of hemoglobin variants based on their migration positions

Jean Riou, Jessica Szuberski, Christian Godart, Henri Wajcman, Jennifer L. Oliveira, James D. Hoyer, Josiane Bardakdjian-Michau

Research output: Contribution to journalArticle

4 Scopus citations

Abstract

Objectives: In this report, we evaluated utility of the capillary electrophoresis (CE) migration position of the CAPILLARYS 2 CE instrument. Methods: The precision of this x-axis number was determined on a selection of common hemoglobin (Hb) variants (Hb S, Hb C, Hb D-Punjab, Hb E, Hb Hope), and the reproducibility of this number was evaluated by comparing the results obtained by two large reference laboratories on 81 Hb variants. Additionally, the CE migration position is given for a total of 409 Hb variants. Results: The x-axis migration position showed excellent intra- and interassay precision. Comparison of Hb variants seen by both laboratories showed that 83% had a difference in migration position of 1 unit or less. Only three rare Hb variants showed a difference of more than 2 units. Conclusion: In summary, the CE migration position is a reproducible value and can be used as an aid in the identification of Hb variants.

Original languageEnglish (US)
Pages (from-to)172-180
Number of pages9
JournalAmerican journal of clinical pathology
Volume149
Issue number2
DOIs
StatePublished - Feb 1 2018

Keywords

  • CAPILLARYS 2
  • Capillary electrophoresis
  • Hemoglobinopathies

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

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