TY - JOUR
T1 - Pre-exposure of human adipose mesenchymal stem cells to soluble factors enhances their homing to brain cancer
AU - Smith, Chris L.
AU - Chaichana, Kaisorn L.
AU - Lee, Young M.
AU - Lin, Benjamin
AU - Stanko, Kevin M.
AU - O’Donnell, Thomas
AU - Gupta, Saksham
AU - Shah, Sagar R.
AU - Wang, Joanne
AU - Wijesekera, Olindi
AU - Delannoy, Michael
AU - Levchenko, Andre
AU - Quiñones-Hinojosa, Alfredo
N1 - Publisher Copyright:
© AlphaMed Press 2015.
PY - 2015
Y1 - 2015
N2 - Recent research advances have established mesenchymal stem cells (MSCs) as a promising vehicle for therapeutic delivery. Their intrinsic tropism for brain injury and brain tumors, their lack of immunogenicity, and their ability to breach the blood-brain barrier make these cells an attractive potential treatment of brain disorders, including brain cancer. Despite these advantages, the efficiency of MSC homing to the brain has been limited in commonly used protocols, hindering the feasibility of such therapies. In the present study, we report a reproducible, comprehensive, cell culture-based approach to enhance human adipose-derived MSC (hAMSC) engraftment to brain tumors. We used micro-and nanotechnological tools to systematically model several steps in the putative homing process. By pre-exposing hAMSCs to glioma-conditioned media and the extracellular matrix proteins fibronectin and laminin,weachieved significant enhancements of the individual homing steps in vitro. This homing was confirmed in an in vivo rodent model of brain cancer. This comprehensive, cellconditioning approach provides a novel method to enhance stem cell homing to gliomas and, potentially, other neurological disorders.
AB - Recent research advances have established mesenchymal stem cells (MSCs) as a promising vehicle for therapeutic delivery. Their intrinsic tropism for brain injury and brain tumors, their lack of immunogenicity, and their ability to breach the blood-brain barrier make these cells an attractive potential treatment of brain disorders, including brain cancer. Despite these advantages, the efficiency of MSC homing to the brain has been limited in commonly used protocols, hindering the feasibility of such therapies. In the present study, we report a reproducible, comprehensive, cell culture-based approach to enhance human adipose-derived MSC (hAMSC) engraftment to brain tumors. We used micro-and nanotechnological tools to systematically model several steps in the putative homing process. By pre-exposing hAMSCs to glioma-conditioned media and the extracellular matrix proteins fibronectin and laminin,weachieved significant enhancements of the individual homing steps in vitro. This homing was confirmed in an in vivo rodent model of brain cancer. This comprehensive, cellconditioning approach provides a novel method to enhance stem cell homing to gliomas and, potentially, other neurological disorders.
KW - Adipose
KW - Bone marrow
KW - Brain tumor
KW - Glioblastoma
KW - Homing
KW - Mesenchymal stem cells
UR - http://www.scopus.com/inward/record.url?scp=84923419456&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84923419456&partnerID=8YFLogxK
U2 - 10.5966/sctm.2014-0149
DO - 10.5966/sctm.2014-0149
M3 - Article
C2 - 25646527
AN - SCOPUS:84923419456
SN - 2157-6564
VL - 4
SP - 239
EP - 251
JO - Stem Cells Translational Medicine
JF - Stem Cells Translational Medicine
IS - 3
ER -