Pre-exposure of human adipose mesenchymal stem cells to soluble factors enhances their homing to brain cancer

Chris L. Smith, Kaisorn L. Chaichana, Young M. Lee, Benjamin Lin, Kevin M. Stanko, Thomas O’Donnell, Saksham Gupta, Sagar R. Shah, Joanne Wang, Olindi Wijesekera, Michael Delannoy, Andre Levchenko, Alfredo Quiñones-Hinojosa

Research output: Contribution to journalArticle

39 Scopus citations

Abstract

Recent research advances have established mesenchymal stem cells (MSCs) as a promising vehicle for therapeutic delivery. Their intrinsic tropism for brain injury and brain tumors, their lack of immunogenicity, and their ability to breach the blood-brain barrier make these cells an attractive potential treatment of brain disorders, including brain cancer. Despite these advantages, the efficiency of MSC homing to the brain has been limited in commonly used protocols, hindering the feasibility of such therapies. In the present study, we report a reproducible, comprehensive, cell culture-based approach to enhance human adipose-derived MSC (hAMSC) engraftment to brain tumors. We used micro-and nanotechnological tools to systematically model several steps in the putative homing process. By pre-exposing hAMSCs to glioma-conditioned media and the extracellular matrix proteins fibronectin and laminin,weachieved significant enhancements of the individual homing steps in vitro. This homing was confirmed in an in vivo rodent model of brain cancer. This comprehensive, cellconditioning approach provides a novel method to enhance stem cell homing to gliomas and, potentially, other neurological disorders.

Original languageEnglish (US)
Pages (from-to)239-251
Number of pages13
JournalStem Cells Translational Medicine
Volume4
Issue number3
DOIs
StatePublished - 2015

Keywords

  • Adipose
  • Bone marrow
  • Brain tumor
  • Glioblastoma
  • Homing
  • Mesenchymal stem cells

ASJC Scopus subject areas

  • Developmental Biology
  • Cell Biology

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    Smith, C. L., Chaichana, K. L., Lee, Y. M., Lin, B., Stanko, K. M., O’Donnell, T., Gupta, S., Shah, S. R., Wang, J., Wijesekera, O., Delannoy, M., Levchenko, A., & Quiñones-Hinojosa, A. (2015). Pre-exposure of human adipose mesenchymal stem cells to soluble factors enhances their homing to brain cancer. Stem Cells Translational Medicine, 4(3), 239-251. https://doi.org/10.5966/sctm.2014-0149