PRDX4, a member of the peroxiredoxin family, is fused to AML1 (RUNX1) in an acute myeloid leukemia patient with a t(X;21)(p22;q22)

Yanming Zhang, Neelmini Emmanuel, Ginny Kamboj, Jianjun Chen, Muhammad Shurafa, Daniel L. Van Dyke, Anne Wiktor, Janet D. Rowley

Research output: Contribution to journalArticle

34 Scopus citations

Abstract

The AML1 gene (also known as RUNX1) at 21 q22 codes for core binding factor (CBF) α, which forms a heterodimer with CBF β that acts as a transcriptional activating factor. CBF is a critical regulator in the generation and differentiation of definitive hematopoietic stem cells and is frequently disrupted in leukemia through chromosome translocations. We cloned a novel AML1 partner gene, PRDX4, in an X;21 translocation in a 74-year-old male patient diagnosed with acute myeloid leukemia-M2. Chromosome analysis detected a t(X;21)(p22;q22) as the sole abnormality in bone marrow samples. The involvement of AML1 was confirmed by fluorescence in situ hybridization studies. Using 3′ RACE-PCR, we cloned a fusion between exon 5 of AML1 and exon 2 of PRDX4. RT-PCR confirmed the fusion and detected another fusion between exon 6 of AML1 and exon 2 of PRDX4, indicating alternative splicing of exon 6 of AML1 in the fusion transcripts. PRDX4 is one of six peroxiredoxin-family genes that are highly conserved in eukaryotes and prokaryotes and are ubiquitously expressed. Peroxiredoxin genes exhibit thioredoxin-dependent peroxidase activity and have been implicated in a number of other cellular functions such as cell proliferation and differentiation. PRDX4 plays a regulatory role in the activation of the transcription factor NF-κB and is significantly down-regulated in acute promyelocytic leukemia. This is the first example of antioxidant enzyme involvement in a chromosome translocation in leukemia.

Original languageEnglish (US)
Pages (from-to)365-370
Number of pages6
JournalGenes Chromosomes and Cancer
Volume40
Issue number4
DOIs
StatePublished - Aug 2004

ASJC Scopus subject areas

  • Genetics
  • Cancer Research

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    Zhang, Y., Emmanuel, N., Kamboj, G., Chen, J., Shurafa, M., Van Dyke, D. L., Wiktor, A., & Rowley, J. D. (2004). PRDX4, a member of the peroxiredoxin family, is fused to AML1 (RUNX1) in an acute myeloid leukemia patient with a t(X;21)(p22;q22). Genes Chromosomes and Cancer, 40(4), 365-370. https://doi.org/10.1002/gcc.20050