Potassium channel openers are uncoupling protonophores: Implication in cardioprotection

Ekhson L. Holmuhamedov, Arshad Jahangir, Andrew Oberlin, Alexander Komarov, Marco Colombini, Andre Terzic

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

Excessive build-up of mitochondrial protonic potential is harmful to cellular homeostasis, and modulation of inner membrane permeability a proposed countermeasure. Here, we demonstrate that structurally distinct potassium channel openers, diazoxide and pinacidil, facilitated transmembrane proton translocation generating H+-selective current through planar phospholipid membrane. Both openers depolarized mitochondria, activated state 4 respiration and reduced oxidative phosphorylation, recapitulating the signature of mitochondrial uncoupling. This effect was maintained in K+-free conditions and shared with the prototypic protonophore 2,4-dinitrophenol. Diazoxide, pinacidil and 2,4-dinitrophenol, but not 2,4-dinitrotoluene lacking protonophoric properties, preserved functional recovery of ischemic heart. The identified protonophoric property of potassium channel openers, thus, implicates a previously unrecognized component in their mechanism of cardioprotection.

Original languageEnglish (US)
Pages (from-to)167-170
Number of pages4
JournalFEBS Letters
Volume568
Issue number1-3
DOIs
StatePublished - Jun 18 2004

Fingerprint

Pinacidil
Diazoxide
2,4-Dinitrophenol
2,4-dinitrotoluene
Potassium Channels
Membranes
Mitochondria
Oxidative Phosphorylation
Protons
Permeability
Phospholipids
Respiration
Homeostasis
Modulation
Recovery

Keywords

  • Cardioprotection
  • Diazoxide
  • Heart
  • Ischemia-reperfusion
  • Mitochondrion
  • Pinacidil
  • Uncoupling

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Holmuhamedov, E. L., Jahangir, A., Oberlin, A., Komarov, A., Colombini, M., & Terzic, A. (2004). Potassium channel openers are uncoupling protonophores: Implication in cardioprotection. FEBS Letters, 568(1-3), 167-170. https://doi.org/10.1016/j.febslet.2004.05.031

Potassium channel openers are uncoupling protonophores : Implication in cardioprotection. / Holmuhamedov, Ekhson L.; Jahangir, Arshad; Oberlin, Andrew; Komarov, Alexander; Colombini, Marco; Terzic, Andre.

In: FEBS Letters, Vol. 568, No. 1-3, 18.06.2004, p. 167-170.

Research output: Contribution to journalArticle

Holmuhamedov, EL, Jahangir, A, Oberlin, A, Komarov, A, Colombini, M & Terzic, A 2004, 'Potassium channel openers are uncoupling protonophores: Implication in cardioprotection', FEBS Letters, vol. 568, no. 1-3, pp. 167-170. https://doi.org/10.1016/j.febslet.2004.05.031
Holmuhamedov, Ekhson L. ; Jahangir, Arshad ; Oberlin, Andrew ; Komarov, Alexander ; Colombini, Marco ; Terzic, Andre. / Potassium channel openers are uncoupling protonophores : Implication in cardioprotection. In: FEBS Letters. 2004 ; Vol. 568, No. 1-3. pp. 167-170.
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