Postnatal expression of myosin isoforms in an expiratory muscle-external abdominal oblique

J. F. Watchko, M. J. Daood, R. L. Vazquez, B. S. Brozanski, W. A. LaFramboise, R. D. Guthrie, Gary C Sieck

Research output: Contribution to journalArticle

23 Citations (Scopus)

Abstract

We studied the postnatal expression of heavy chain (MHC) and native myosin isoforms in an expiratory abdominal muscle of the rat, the external abdominal oblique (EO). Moreover, we contrasted EO myosin expression with that of the costal diaphragm (DIA) to draw inspiratory vs. expiratory muscle comparisons during development. Examination of MHC gels demonstrated a mature phenotype of slow and adult fast myosin isoforms at an earlier age in the EO (day 60) than in the DIA [day > 115 (adult)]. The mature MHC phenotype of the EO was characterized by a preponderance of MHC 2B, whereas the DIA was characterized by approximately equal portions of MHC slow, MHC 2A, and MHC 2X. During early postnatal development, there was a delay in the expression of MHC 2A in the EO compared with the DIA. However, MHC 2B, expressed later in development in both muscles, was noted in the EO before the DIA. We conclude that 1) the EO mature myosin phenotype is characterized by a preponderance of fast myosin isoforms and 2) the EO and DIA muscles are subject to different temporal patterns of isoform expression during postnatal development.

Original languageEnglish (US)
Pages (from-to)1860-1866
Number of pages7
JournalJournal of Applied Physiology
Volume73
Issue number5
StatePublished - 1992
Externally publishedYes

Fingerprint

Myosins
Diaphragm
Protein Isoforms
Phenotype
Abdominal Muscles
Muscles
Abdominal Oblique Muscles
Gels

Keywords

  • development
  • diaphragm
  • newborn
  • respiratory muscles

ASJC Scopus subject areas

  • Endocrinology
  • Physiology
  • Orthopedics and Sports Medicine
  • Physical Therapy, Sports Therapy and Rehabilitation

Cite this

Watchko, J. F., Daood, M. J., Vazquez, R. L., Brozanski, B. S., LaFramboise, W. A., Guthrie, R. D., & Sieck, G. C. (1992). Postnatal expression of myosin isoforms in an expiratory muscle-external abdominal oblique. Journal of Applied Physiology, 73(5), 1860-1866.

Postnatal expression of myosin isoforms in an expiratory muscle-external abdominal oblique. / Watchko, J. F.; Daood, M. J.; Vazquez, R. L.; Brozanski, B. S.; LaFramboise, W. A.; Guthrie, R. D.; Sieck, Gary C.

In: Journal of Applied Physiology, Vol. 73, No. 5, 1992, p. 1860-1866.

Research output: Contribution to journalArticle

Watchko, JF, Daood, MJ, Vazquez, RL, Brozanski, BS, LaFramboise, WA, Guthrie, RD & Sieck, GC 1992, 'Postnatal expression of myosin isoforms in an expiratory muscle-external abdominal oblique', Journal of Applied Physiology, vol. 73, no. 5, pp. 1860-1866.
Watchko JF, Daood MJ, Vazquez RL, Brozanski BS, LaFramboise WA, Guthrie RD et al. Postnatal expression of myosin isoforms in an expiratory muscle-external abdominal oblique. Journal of Applied Physiology. 1992;73(5):1860-1866.
Watchko, J. F. ; Daood, M. J. ; Vazquez, R. L. ; Brozanski, B. S. ; LaFramboise, W. A. ; Guthrie, R. D. ; Sieck, Gary C. / Postnatal expression of myosin isoforms in an expiratory muscle-external abdominal oblique. In: Journal of Applied Physiology. 1992 ; Vol. 73, No. 5. pp. 1860-1866.
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