Postnatal downregulation of inhibitory neuromuscular transmission to the longitudinal muscle of the guinea pig ileum

X. Bian, J. E. Burda, M. Carrasquillo, J. J. Galligan

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

Neuromuscular transmission is crucial for normal gut motility but little is known about its postnatal maturation. This study investigated excitatory-inhibitory neuromuscular transmission in vitro using ileal nerve-muscle preparations made from neonatal (≤48 h postnatal) and adult (∼4 months postnatal) guinea pigs. In tissues from neonates and adults, nicotine (0.3-30 μmol L-1) contracted longitudinal muscle preparations in a tetrodotoxin (TTX) (0.3 μmol L-1)-sensitive manner. The muscarinic receptor antagonist, scopolamine (1 μmol L -1), reduced substantially nicotine-induced contractions in neonatal tissues but not adult tissues. In the presence of N-nitro-l-arginine (NLA, 100 μmol L-1) to block nitric oxide (NO) mediated inhibitory neuromuscular transmission, scopolamine-resistant nicotine-induced contractions were revealed in neonatal tissues. NLA enhanced the nicotine-induced contractions in neonatal but not in adult tissues. Electrical field stimulation (20 V; 0.3 ms; 5-25 Hz, scopolamine 1 μmol L-1 present) caused NLA and TTX-sensitive longitudinal muscle relaxations. Frequency-response curves in neonatal tissues were left-shifted compared with those obtained in adult tissues. Immunohistochemical studies revealed that NO synthase (NOS)-immunoreactivity (ir) was present in nerve fibres supplying the longitudinal muscle in neonatal and adult tissues. However, quantitative studies demonstrated that fluorescence intensity of NOS-ir nerve fibres was higher in neonatal than adult tissues. Nerve fibres containing substance P were abundant in longitudinal muscle in adult but not in neonatal tissues. Inhibitory neuromuscular transmission is relatively more effective in the neonatal guinea pig small intestine. Delayed maturation of excitatory motor pathways might contribute to paediatric motility disturbances.

Original languageEnglish (US)
Pages (from-to)969-977
Number of pages9
JournalNeurogastroenterology and Motility
Volume21
Issue number9
DOIs
StatePublished - Sep 1 2009

Keywords

  • Autonomic neurotransmission
  • Enteric nervous system
  • Postnatal development

ASJC Scopus subject areas

  • Physiology
  • Endocrine and Autonomic Systems
  • Gastroenterology

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