Possible overestimation of penicillin resistant Streptococcus pneumoniae colonization rates due to misidentification of oropharyngeal streptococci

C. William Wester, Deepak Ariga, Catherine Nathan, Thomas W. Rice, Joseph Pulvirenti, Robin Patel, Frank Kocka, Joanna Ortiz, Robin Patel

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Standard identification of Streptococcus pneumoniae by optochin and bile solubility testing can lead to ambiguous results for certain isolates. Newer bacteriologic identification techniques (e.g., DNA probes) now exist. In a prospective point prevalence study of oropharyngeal S. pneumoniae carriage rates among outpatients, we compared standard organism identification techniques to DNA probe testing. By standard identification criteria, 35 (4%) of 872 isolates were characterized as presumptive S. pneumoniae. Thirty of 35 presumptive isolates were recoverable for DNA probing; 9 (30%) presumptive isolates were confirmed using a DNA probe. The antimicrobial susceptibility pattern of these DNA probe positive isolates closely paralleled that of clinical blood isolates of S. pneumoniae obtained during the study period. The 21 (70%) DNA probe negative isolates, which may represent phylogenetically related species (such as S. mitis or S. oralis), had significantly reduced antimicrobial susceptibility patterns when compared with the DNA probe positive isolates. In colonization studies, if classic criteria (optochin disc zone and bile solubility) are the sole means of identification, S. pneumoniae penicillin resistance rates may be over-reported.

Original languageEnglish (US)
Pages (from-to)263-268
Number of pages6
JournalDiagnostic Microbiology and Infectious Disease
Volume42
Issue number4
DOIs
StatePublished - 2002

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DNA Probes
Streptococcus pneumoniae
Streptococcus
Penicillins
Bile
Solubility
Bacteriological Techniques
Penicillin Resistance
Outpatients
Cross-Sectional Studies
DNA

ASJC Scopus subject areas

  • Infectious Diseases
  • Immunology and Allergy
  • Virology
  • Parasitology
  • Microbiology
  • Immunology
  • Applied Microbiology and Biotechnology

Cite this

Possible overestimation of penicillin resistant Streptococcus pneumoniae colonization rates due to misidentification of oropharyngeal streptococci. / Wester, C. William; Ariga, Deepak; Nathan, Catherine; Rice, Thomas W.; Pulvirenti, Joseph; Patel, Robin; Kocka, Frank; Ortiz, Joanna; Patel, Robin.

In: Diagnostic Microbiology and Infectious Disease, Vol. 42, No. 4, 2002, p. 263-268.

Research output: Contribution to journalArticle

Wester, C. William ; Ariga, Deepak ; Nathan, Catherine ; Rice, Thomas W. ; Pulvirenti, Joseph ; Patel, Robin ; Kocka, Frank ; Ortiz, Joanna ; Patel, Robin. / Possible overestimation of penicillin resistant Streptococcus pneumoniae colonization rates due to misidentification of oropharyngeal streptococci. In: Diagnostic Microbiology and Infectious Disease. 2002 ; Vol. 42, No. 4. pp. 263-268.
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