TY - JOUR
T1 - Porous tantalum and poly-ε-caprolactone biocomposites for osteochondral defect repair
T2 - Preliminary studies in rabbits
AU - Mrosek, Eike H.
AU - Schagemann, Jan C.
AU - Chung, Hsi Wei
AU - Fitzsimmons, James S.
AU - Yaszemski, Michael J.
AU - Mardones, Rodrigo M.
AU - O'Driscoll, Shawn W.
AU - Reinholz, Gregory G.
PY - 2010/2
Y1 - 2010/2
N2 - Currently, various techniques are in use for the repair of osteochondral defects, none of them being truly satisfactory and they are often two step procedures. Comorbidity due to cancellous bone harvest from the iliac crest further complicates the procedure. Our previous in vitro studies suggest that porous tantalum (TM) or poly-ε-caprolactone scaffolds (PCL) in combination with periosteal grafts could be used for osteochondral defect repair. In this in vivo study, cylindrical osteochondral defects were created on the medial and lateral condyles of 10 rabbits and filled with TM/periosteum or PCL/periosteum biosynthetic composites (n = 8 each). The regenerated osteochondral tissue was then analyzed histologically, and evaluated in an independent and blinded manner by five different observers using a 30-point histological score. The overall histological score for PCL/periosteum was significantly better than for TM/periosteum. However, most of the regenerates were well integrated with the surrounding bone (PCL/periosteum, n = 6.4; TM/periosteum, n = 7) along with partial restoration of the tidemark (PCL/periosteum, n = 4.4; TM/periosteum, n = 5.6). A cover of hyaline-like morphology was found after PCL/periosteum treatment (n = 4.8), yet the cartilage yields were inconsistent. In conclusion, the applied TM and PCL scaffolds promoted excellent subchondral bone regeneration. Neo-cartilage formation from periosteum supported by a scaffold was inconsistent. This is the first study to show in vivo results of both PCL andTMscaffolds for a novel approach to osteochondral defect repair.
AB - Currently, various techniques are in use for the repair of osteochondral defects, none of them being truly satisfactory and they are often two step procedures. Comorbidity due to cancellous bone harvest from the iliac crest further complicates the procedure. Our previous in vitro studies suggest that porous tantalum (TM) or poly-ε-caprolactone scaffolds (PCL) in combination with periosteal grafts could be used for osteochondral defect repair. In this in vivo study, cylindrical osteochondral defects were created on the medial and lateral condyles of 10 rabbits and filled with TM/periosteum or PCL/periosteum biosynthetic composites (n = 8 each). The regenerated osteochondral tissue was then analyzed histologically, and evaluated in an independent and blinded manner by five different observers using a 30-point histological score. The overall histological score for PCL/periosteum was significantly better than for TM/periosteum. However, most of the regenerates were well integrated with the surrounding bone (PCL/periosteum, n = 6.4; TM/periosteum, n = 7) along with partial restoration of the tidemark (PCL/periosteum, n = 4.4; TM/periosteum, n = 5.6). A cover of hyaline-like morphology was found after PCL/periosteum treatment (n = 4.8), yet the cartilage yields were inconsistent. In conclusion, the applied TM and PCL scaffolds promoted excellent subchondral bone regeneration. Neo-cartilage formation from periosteum supported by a scaffold was inconsistent. This is the first study to show in vivo results of both PCL andTMscaffolds for a novel approach to osteochondral defect repair.
KW - Cartilage engineering
KW - Neocartilage
KW - Osteochondral defects
KW - Osteochondrosis dissecans
KW - Poly-ε-caprolactone
KW - Scaffolds
KW - Tantalum
KW - Trabecular metal
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U2 - 10.1002/jor.20983
DO - 10.1002/jor.20983
M3 - Article
C2 - 19743507
AN - SCOPUS:73849093929
SN - 0736-0266
VL - 28
SP - 141
EP - 148
JO - Journal of Orthopaedic Research
JF - Journal of Orthopaedic Research
IS - 2
ER -