Porous chitosan-chondroitin sulfate scaffolds to mimic the prostate cancer microenvironment

Kailei Xu, Kavya Ganapathy, Zi Wang, John A III Copland, Ratna Chakrabarti, Stephen J. Florczyk

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: Researchers have shown that 3D cultures better mimic the in vivo tumor microenvironment than 2D cultures [1]. Therefore, 3D culture of cancer cells using 3D scaffolds has been investigated to explore the interaction between cells and stroma. Composition of the 3D substrate influences cell function, signaling, and cell morphology [2]. Chitosan (C) is a commonly used biomaterial that has beneficial biological properties including hydrophilic surface, biodegradability, and biocompatibility [3]. Due to its cationic nature, chitosan can form an ionic complex by mixing with anionic polymers. Chondroitin sulfate (CS) is one of glycosaminoglycans in proteoglycans [4]. CS was found in greater amounts in advanced and metastatic prostate cancer than early stage [5]. In this study, novel 3D porous C-CS scaffolds were prepared by mixing chitosan with CS and three scaffold compositions were prepared with different CS concentrations: 0.1 wt% (4-0.1), 0.5 wt% (4-0.5) and 1 wt% (4-1) CS. The effect of CS composition in scaffolds on prostate cancer (PCa) cells was investigated by in vitro analysis with PC-3 and 22Rv1 cell lines. Our hypothesis is that PCa cultures will show higher expression of cytokeratin 8 (KRT8), androgen receptor (AR), prostate specific antigen (PSA), and LIM domain kinase 1 (LIMK1) in scaffolds with higher CS concentration.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

Fingerprint

Tumor Microenvironment
Chondroitin Sulfates
Chitosan
Scaffolds
Prostatic Neoplasms
Cells
Cell culture
Lim Kinases
Chemical analysis
Keratin-8
chitosan sulfate
Sulfates
Biodegradability
Androgen Receptors
Biocompatible Materials
Proteoglycans
Scaffolds (biology)
Prostate-Specific Antigen
Antigens
Glycosaminoglycans

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

Cite this

Xu, K., Ganapathy, K., Wang, Z., Copland, J. A. III., Chakrabarti, R., & Florczyk, S. J. (2019). Porous chitosan-chondroitin sulfate scaffolds to mimic the prostate cancer microenvironment. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.

Porous chitosan-chondroitin sulfate scaffolds to mimic the prostate cancer microenvironment. / Xu, Kailei; Ganapathy, Kavya; Wang, Zi; Copland, John A III; Chakrabarti, Ratna; Florczyk, Stephen J.

Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Xu, K, Ganapathy, K, Wang, Z, Copland, JAIII, Chakrabarti, R & Florczyk, SJ 2019, Porous chitosan-chondroitin sulfate scaffolds to mimic the prostate cancer microenvironment. in Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium, vol. 40, Society for Biomaterials, 42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence, Seattle, United States, 4/3/19.
Xu K, Ganapathy K, Wang Z, Copland JAIII, Chakrabarti R, Florczyk SJ. Porous chitosan-chondroitin sulfate scaffolds to mimic the prostate cancer microenvironment. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials. 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
Xu, Kailei ; Ganapathy, Kavya ; Wang, Zi ; Copland, John A III ; Chakrabarti, Ratna ; Florczyk, Stephen J. / Porous chitosan-chondroitin sulfate scaffolds to mimic the prostate cancer microenvironment. Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting. Society for Biomaterials, 2019. (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium).
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