Porous chitosan-chondroitin sulfate scaffolds to mimic the prostate cancer microenvironment

Kailei Xu, Kavya Ganapathy, Zi Wang, John A III Copland, Ratna Chakrabarti, Stephen J. Florczyk

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Statement of Purpose: Researchers have shown that 3D cultures better mimic the in vivo tumor microenvironment than 2D cultures [1]. Therefore, 3D culture of cancer cells using 3D scaffolds has been investigated to explore the interaction between cells and stroma. Composition of the 3D substrate influences cell function, signaling, and cell morphology [2]. Chitosan (C) is a commonly used biomaterial that has beneficial biological properties including hydrophilic surface, biodegradability, and biocompatibility [3]. Due to its cationic nature, chitosan can form an ionic complex by mixing with anionic polymers. Chondroitin sulfate (CS) is one of glycosaminoglycans in proteoglycans [4]. CS was found in greater amounts in advanced and metastatic prostate cancer than early stage [5]. In this study, novel 3D porous C-CS scaffolds were prepared by mixing chitosan with CS and three scaffold compositions were prepared with different CS concentrations: 0.1 wt% (4-0.1), 0.5 wt% (4-0.5) and 1 wt% (4-1) CS. The effect of CS composition in scaffolds on prostate cancer (PCa) cells was investigated by in vitro analysis with PC-3 and 22Rv1 cell lines. Our hypothesis is that PCa cultures will show higher expression of cytokeratin 8 (KRT8), androgen receptor (AR), prostate specific antigen (PSA), and LIM domain kinase 1 (LIMK1) in scaffolds with higher CS concentration.

Original languageEnglish (US)
Title of host publicationSociety for Biomaterials Annual Meeting and Exposition 2019
Subtitle of host publicationThe Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting
PublisherSociety for Biomaterials
Number of pages1
ISBN (Electronic)9781510883901
StatePublished - Jan 1 2019
Event42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Seattle, United States
Duration: Apr 3 2019Apr 6 2019

Publication series

NameTransactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium
Volume40
ISSN (Print)1526-7547

Conference

Conference42nd Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence
CountryUnited States
CitySeattle
Period4/3/194/6/19

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ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Biotechnology
  • Biomaterials
  • Materials Chemistry

Cite this

Xu, K., Ganapathy, K., Wang, Z., Copland, J. A. III., Chakrabarti, R., & Florczyk, S. J. (2019). Porous chitosan-chondroitin sulfate scaffolds to mimic the prostate cancer microenvironment. In Society for Biomaterials Annual Meeting and Exposition 2019: The Pinnacle of Biomaterials Innovation and Excellence - Transactions of the 42nd Annual Meeting (Transactions of the Annual Meeting of the Society for Biomaterials and the Annual International Biomaterials Symposium; Vol. 40). Society for Biomaterials.