Porcine ex vivo liver phantom for dynamic contrast-enhanced computed tomography: Development and initial results

Scott M. Thompson, Juan C. Ramirez-Giraldo, Bruce Knudsen, Joseph Peter Grande, Jodie A. Christner, Man Xu, David A Woodrum, Cynthia H McCollough, Matthew R Callstrom

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objectives: To demonstrate the feasibility of developing a fixed, dual-input, biologic liver phantom for dynamic contrast-enhanced computed tomography (CT) imaging and to report initial results of use of the phantom for quantitative CT perfusion imaging. Materials and Methods: Porcine livers were obtained from completed surgical studies and perfused with saline and fixative. The phantom was placed in a body-shaped, CT-compatible acrylic container and connected to a perfusion circuit fitted with a contrast injection port. Flow-controlled contrast-enhanced imaging experiments were performed using 128-slice and 64-slice dual-source multidetector CT scanners. CT angiography protocols were used to obtain portal venous and hepatic arterial vascular enhancement, reproduced over a period of 4 to 6 months. CT perfusion protocols were used at different input flow rates to correlate input flow with calculated tissue perfusion, to test reproducibility, and to determine the feasibility of simultaneous dual-input liver perfusion. Histologic analysis of the liver phantom was also performed. Results: CT angiogram 3-dimensional reconstructions demonstrated homogenous tertiary and quaternary branching of the portal venous system to the periphery of all lobes of the liver as well as enhancement of the hepatic arterial system to all lobes of the liver and gallbladder throughout the study period. For perfusion CT, the correlation between the calculated mean tissue perfusion in a volume of interest and input pump flow rate was excellent (R = 0.996) and color blood flow maps demonstrated variations in regional perfusion in a narrow range. Repeat perfusion CT experiments demonstrated reproducible time-attenuation curves, and dual-input perfusion CT experiments demonstrated that simultaneous dual input liver perfusion is feasible. Histologic analysis demonstrated that the hepatic microvasculature and architecture appeared intact and well preserved at the completion of 4 to 6 months of laboratory experiments and contrast-enhanced imaging. Conclusions: We have demonstrated successful development of a porcine liver phantom using a flow-controlled extracorporeal perfusion circuit. This phantom exhibited reproducible dynamic contrast-enhanced CT of the hepatic arterial and portal venous system over a 4- to 6-month period.

Original languageEnglish (US)
Pages (from-to)586-593
Number of pages8
JournalInvestigative Radiology
Volume46
Issue number9
DOIs
StatePublished - Sep 2011

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Swine
Tomography
Perfusion
Liver
Portal System
Fixatives
Perfusion Imaging
Multidetector Computed Tomography
Microvessels
Gallbladder
Blood Vessels
Angiography
Color
Injections

Keywords

  • biologic phantom
  • computed tomography
  • perfusion imaging
  • porcine

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging

Cite this

Porcine ex vivo liver phantom for dynamic contrast-enhanced computed tomography : Development and initial results. / Thompson, Scott M.; Ramirez-Giraldo, Juan C.; Knudsen, Bruce; Grande, Joseph Peter; Christner, Jodie A.; Xu, Man; Woodrum, David A; McCollough, Cynthia H; Callstrom, Matthew R.

In: Investigative Radiology, Vol. 46, No. 9, 09.2011, p. 586-593.

Research output: Contribution to journalArticle

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AU - Thompson, Scott M.

AU - Ramirez-Giraldo, Juan C.

AU - Knudsen, Bruce

AU - Grande, Joseph Peter

AU - Christner, Jodie A.

AU - Xu, Man

AU - Woodrum, David A

AU - McCollough, Cynthia H

AU - Callstrom, Matthew R

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