Polysome analysis for determining mRNA and ribosome association in saccharomyces cerevisiae

Wenqian Hu, Jeff Coller

Research output: Chapter in Book/Report/Conference proceedingChapter

1 Scopus citations

Abstract

The control of mRNA translation is vital for gene expression and it is regulated under various physiological and pathological conditions (Groppo and Richter, 2009). For example, under some physiological conditions, translational initiation is impaired. Therefore, the association of mRNA with ribosomes and/or ribosomal subunits can provide a powerful means to dissect specific aspects of posttranscriptional mRNA regulation. This protocol describes a technique used to determine ribosome occupancy on mRNA.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationRNA
PublisherAcademic Press Inc
Pages193-206
Number of pages14
ISBN (Print)9780124200371
DOIs
StatePublished - 2013

Publication series

NameMethods in Enzymology
Volume530
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

Keywords

  • Cell lysis
  • Grow yeast cells
  • Polysome analysis
  • Sedimentation analysis
  • Sucrose gradient

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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