Polymeric delivery vehicles for bone growth factors

Lichun Lu, Susan J. Peter, Georgios N. Stamatas, Antonios G. Mikos

Research output: Contribution to journalArticle

Abstract

Recombinant human transforming growth factor-β1 (TGF-β1) was incorporated into biodegradable microparticles of blends of poly(DL-lactic-co-glycolic acid) (PLGA) and poly(ethylene glycol) (PEG) at 6 ng per mg microparticle. Fluorescein isothiocynate-labelled bovine serum albumin (FITC-BSA) was co-encapsulated as a porogen. The effects of PEG content (0, 1, or 5 wt%) and buffer pH (3, 5, or 7.4) on TGF-β1 release kinetics and PLGA degradation were determined in vitro for up to 28 days. TGF-β1 was released in a multi-phasic fashion including an initial burst effect. Increasing the PEG content resulted in decreased cumulative mass of released proteins. Aggregation of FITC-BSA occurred at acidic buffer pH, which led to decreased protein release rates. PLGA degradation was also enhanced at 5% PEG, which was significantly accelerated at acidic pH conditions. Co-encapsulation of TGF-β1 with FITC-Dextran reduced the initial burst effect as compared to FITC-BSA. The TGF-β1 released from PLGA/PEG microparticles enhanced the proliferation and osteoblastic differentiation of marrow stromal cells cultured on poly(propylene fumarate) (PPF) substrates. The cells showed significantly increased total cell number, alkaline phosphatase (ALPase) activity, and osteocalcin production after 21 days, as compared to cells cultured under control conditions without TGF-β1. These results suggest that PLGA/PEG blend microparticles can serve as delivery vehicles for controlled release of TGF-β1, which may find applications in modulating cellular response during bone healing at a skeletal defect site.

Original languageEnglish (US)
Pages (from-to)124-138
Number of pages15
JournalACS Symposium Series
Volume752
StatePublished - 2000
Externally publishedYes

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glycolic acid
Transforming Growth Factors
Intercellular Signaling Peptides and Proteins
Bone
Polyethylene glycols
Bovine Serum Albumin
Fluorescein
Acids
Buffers
Proteins
Degradation
Dextran
Osteocalcin
Phosphatases
Encapsulation
Alkaline Phosphatase
Polypropylenes
Agglomeration
polylactic acid-polyglycolic acid copolymer
Milk

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Lu, L., Peter, S. J., Stamatas, G. N., & Mikos, A. G. (2000). Polymeric delivery vehicles for bone growth factors. ACS Symposium Series, 752, 124-138.

Polymeric delivery vehicles for bone growth factors. / Lu, Lichun; Peter, Susan J.; Stamatas, Georgios N.; Mikos, Antonios G.

In: ACS Symposium Series, Vol. 752, 2000, p. 124-138.

Research output: Contribution to journalArticle

Lu, L, Peter, SJ, Stamatas, GN & Mikos, AG 2000, 'Polymeric delivery vehicles for bone growth factors', ACS Symposium Series, vol. 752, pp. 124-138.
Lu L, Peter SJ, Stamatas GN, Mikos AG. Polymeric delivery vehicles for bone growth factors. ACS Symposium Series. 2000;752:124-138.
Lu, Lichun ; Peter, Susan J. ; Stamatas, Georgios N. ; Mikos, Antonios G. / Polymeric delivery vehicles for bone growth factors. In: ACS Symposium Series. 2000 ; Vol. 752. pp. 124-138.
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