Polymerase chain reaction-based microsatellite analysis of fine-needle aspirations from Hurthle cell neoplasms

Yumi Takiyama, Motoyasu Saji, Douglas P. Clark, Grace S. Phillips, Dorry L. Segev, Robert Christian Smallridge, William H. Westra, Robert Udelsman, Martha A. Zeiger

Research output: Contribution to journalArticle

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Abstract

Fine-needle aspiration (FNA) of the thyroid is the sine qua non in the preoperative evaluation of thyroid nodules. Despite this, cytological examination of FNA cannot differentiate malignant from benign Hurthle cell neoplasms. We have previously shown that Hurthle cell carcinomas harbor more genetic alterations on chromosomal arms 1q and 2p than Hurthle cell adenomas, and that all Hurthle cell neoplasms have a significantly higher frequency of alterations on chromosomal arm 1p compared with normal thyroid. To determine if these genetic alterations could be detected in FNA samples, we examined DNA from FNAs that were available from eight Hurthle cell neoplasms. Polymerase chain reaction (PCR) amplification of DNA demonstrated either direct correlation with alterations seen in the tumor samples or in some instances, additional chromosomal alterations. We conclude that PCR-based microsatellite DNA analysis of preoperative FNA samples from Hurthle cell neoplasms can potentially distinguish Hurthle cell carcinomas from adenomas and that with further validation and perfection, this technique may allow more optimal surgical management of patients with these lesions.

Original languageEnglish (US)
Pages (from-to)853-857
Number of pages5
JournalThyroid
Volume7
Issue number6
StatePublished - 1997

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Oxyphil Cells
Fine Needle Biopsy
Microsatellite Repeats
Polymerase Chain Reaction
Neoplasms
Adenoma
DNA
Thyroid Gland
Carcinoma
Thyroid Nodule

ASJC Scopus subject areas

  • Endocrinology

Cite this

Takiyama, Y., Saji, M., Clark, D. P., Phillips, G. S., Segev, D. L., Smallridge, R. C., ... Zeiger, M. A. (1997). Polymerase chain reaction-based microsatellite analysis of fine-needle aspirations from Hurthle cell neoplasms. Thyroid, 7(6), 853-857.

Polymerase chain reaction-based microsatellite analysis of fine-needle aspirations from Hurthle cell neoplasms. / Takiyama, Yumi; Saji, Motoyasu; Clark, Douglas P.; Phillips, Grace S.; Segev, Dorry L.; Smallridge, Robert Christian; Westra, William H.; Udelsman, Robert; Zeiger, Martha A.

In: Thyroid, Vol. 7, No. 6, 1997, p. 853-857.

Research output: Contribution to journalArticle

Takiyama, Y, Saji, M, Clark, DP, Phillips, GS, Segev, DL, Smallridge, RC, Westra, WH, Udelsman, R & Zeiger, MA 1997, 'Polymerase chain reaction-based microsatellite analysis of fine-needle aspirations from Hurthle cell neoplasms', Thyroid, vol. 7, no. 6, pp. 853-857.
Takiyama, Yumi ; Saji, Motoyasu ; Clark, Douglas P. ; Phillips, Grace S. ; Segev, Dorry L. ; Smallridge, Robert Christian ; Westra, William H. ; Udelsman, Robert ; Zeiger, Martha A. / Polymerase chain reaction-based microsatellite analysis of fine-needle aspirations from Hurthle cell neoplasms. In: Thyroid. 1997 ; Vol. 7, No. 6. pp. 853-857.
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abstract = "Fine-needle aspiration (FNA) of the thyroid is the sine qua non in the preoperative evaluation of thyroid nodules. Despite this, cytological examination of FNA cannot differentiate malignant from benign Hurthle cell neoplasms. We have previously shown that Hurthle cell carcinomas harbor more genetic alterations on chromosomal arms 1q and 2p than Hurthle cell adenomas, and that all Hurthle cell neoplasms have a significantly higher frequency of alterations on chromosomal arm 1p compared with normal thyroid. To determine if these genetic alterations could be detected in FNA samples, we examined DNA from FNAs that were available from eight Hurthle cell neoplasms. Polymerase chain reaction (PCR) amplification of DNA demonstrated either direct correlation with alterations seen in the tumor samples or in some instances, additional chromosomal alterations. We conclude that PCR-based microsatellite DNA analysis of preoperative FNA samples from Hurthle cell neoplasms can potentially distinguish Hurthle cell carcinomas from adenomas and that with further validation and perfection, this technique may allow more optimal surgical management of patients with these lesions.",
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AU - Takiyama, Yumi

AU - Saji, Motoyasu

AU - Clark, Douglas P.

AU - Phillips, Grace S.

AU - Segev, Dorry L.

AU - Smallridge, Robert Christian

AU - Westra, William H.

AU - Udelsman, Robert

AU - Zeiger, Martha A.

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N2 - Fine-needle aspiration (FNA) of the thyroid is the sine qua non in the preoperative evaluation of thyroid nodules. Despite this, cytological examination of FNA cannot differentiate malignant from benign Hurthle cell neoplasms. We have previously shown that Hurthle cell carcinomas harbor more genetic alterations on chromosomal arms 1q and 2p than Hurthle cell adenomas, and that all Hurthle cell neoplasms have a significantly higher frequency of alterations on chromosomal arm 1p compared with normal thyroid. To determine if these genetic alterations could be detected in FNA samples, we examined DNA from FNAs that were available from eight Hurthle cell neoplasms. Polymerase chain reaction (PCR) amplification of DNA demonstrated either direct correlation with alterations seen in the tumor samples or in some instances, additional chromosomal alterations. We conclude that PCR-based microsatellite DNA analysis of preoperative FNA samples from Hurthle cell neoplasms can potentially distinguish Hurthle cell carcinomas from adenomas and that with further validation and perfection, this technique may allow more optimal surgical management of patients with these lesions.

AB - Fine-needle aspiration (FNA) of the thyroid is the sine qua non in the preoperative evaluation of thyroid nodules. Despite this, cytological examination of FNA cannot differentiate malignant from benign Hurthle cell neoplasms. We have previously shown that Hurthle cell carcinomas harbor more genetic alterations on chromosomal arms 1q and 2p than Hurthle cell adenomas, and that all Hurthle cell neoplasms have a significantly higher frequency of alterations on chromosomal arm 1p compared with normal thyroid. To determine if these genetic alterations could be detected in FNA samples, we examined DNA from FNAs that were available from eight Hurthle cell neoplasms. Polymerase chain reaction (PCR) amplification of DNA demonstrated either direct correlation with alterations seen in the tumor samples or in some instances, additional chromosomal alterations. We conclude that PCR-based microsatellite DNA analysis of preoperative FNA samples from Hurthle cell neoplasms can potentially distinguish Hurthle cell carcinomas from adenomas and that with further validation and perfection, this technique may allow more optimal surgical management of patients with these lesions.

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