Polyethylene glycol induces apoptosis in HT-29 cells: Potential mechanism for chemoprevention of colon cancer

Hemant K. Roy, John K. Dibaise, John Black, William J. Karolski, Anne Ratashak, Sajid Ansari

Research output: Contribution to journalArticle

31 Scopus citations

Abstract

Recent experimental evidence suggests that polyethylene glycol (PEG) is a highly effective chemopreventive agent against colon cancer; however, the mechanism(s) remain largely unexplored. To further elucidate this issue, we evaluated the effect of PEG on two human colon cancer cell lines. PEG treatment resulted in a dose- and time-dependent reduction in cell number without alteration in markers of cell proliferation. However, there was a dramatic and specific, concentration-dependent induction of apoptosis, with 50 mM PEG rendering approximately half the cells apoptotic. This corresponded with a 17-fold induction in the expression of the pro-apoptotic protein, prostate apoptosis response-4. Our data suggest that induction of apoptosis may be responsible, at least in part, for the ability of PEG to prevent experimental colon cancer.

Original languageEnglish (US)
Pages (from-to)143-146
Number of pages4
JournalFEBS Letters
Volume496
Issue number2-3
DOIs
StatePublished - May 11 2001

Keywords

  • Apoptosis
  • Chemoprevention
  • Colon cancer
  • Polyethylene glycol

ASJC Scopus subject areas

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

Fingerprint Dive into the research topics of 'Polyethylene glycol induces apoptosis in HT-29 cells: Potential mechanism for chemoprevention of colon cancer'. Together they form a unique fingerprint.

  • Cite this

    Roy, H. K., Dibaise, J. K., Black, J., Karolski, W. J., Ratashak, A., & Ansari, S. (2001). Polyethylene glycol induces apoptosis in HT-29 cells: Potential mechanism for chemoprevention of colon cancer. FEBS Letters, 496(2-3), 143-146. https://doi.org/10.1016/S0014-5793(01)02420-6