Poly(ethylene glycol) hydrogel microstructures encapsulating living cells

Won Gun Koh, Alexander Revzin, Michael V. Pishko

Research output: Contribution to journalArticle

210 Scopus citations

Abstract

We present an easy and effective method for the encapsulation of cells inside PEG-based hydrogel microstructures fabricated using photolithography. High-density arrays of three-dimensional microstructures were created on substrates using this method. Mammalian cells were encapsulated in cylindrical hydrogel microstructures of 600 and 50 μm in diameter or in cubic hydrogel structures in microfluidic channels. Reducing lateral dimension of the individual hydrogel microstructure to 50 μm allowed us to isolate 1-3 cells per microstructure. Viability assays demonstrated that cells remained viable inside these hydrogels after encapsulation for up to 7 days.

Original languageEnglish (US)
Pages (from-to)2459-2462
Number of pages4
JournalLangmuir
Volume18
Issue number7
DOIs
StatePublished - Apr 2 2002

ASJC Scopus subject areas

  • Materials Science(all)
  • Condensed Matter Physics
  • Surfaces and Interfaces
  • Spectroscopy
  • Electrochemistry

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