Polyclonal activation of the murine immune system by an antibody to IgD. V. Effect on germinal centers

Thomas J Flotte, F. D. Finkelman, G. J. Thorbecke

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Heterologous anti-mouse δ chain has been shown to induce T-independent polyclonal B cell proliferation by 24 h after injection into mice and a T-dependent polyclonal increase in the number of IgG-secreting cells in spleen and lymph nodes 6 days after injection. The effect of anti-δ on germinal center cells has been difficult to determine, however, since anti-δ-induced blast transformation of mantle zone cells makes it difficult to distinguish these cells from germinal center cells. To clarify the effect of anti-δ on germinal centers, we stained lymph node sections from control and anti-δ-injected mice with peanut agglutinin conjugated to horseradish peroxidase which clearly differentiates the germinal center cells from other large and small lymphoid cells. For the first 5 days after i.v. injection of goat anti-mouse δ, the germinal center volumes did not differ significantly from those of mice injected with control antibodies. On days 6 and 7 after injection, the lymph node germinal center volumes of anti-δ-treated mice increased on average over 10-fold as compared to those of mice injected with an equal amount of control antibody. Thus, while anti-δ antibody directly induced blastogenesis of mantle layer B cells, it appears to have little direct effect on germinal center cells. In contrast, rapid germinal center development is seen in anti-δ-injected mice during the T-dependent phase of B cell activation and occurs simultaneously with the appearance of large numbers of IgG-secreting cells.

Original languageEnglish (US)
Pages (from-to)725-728
Number of pages4
JournalEuropean Journal of Immunology
Volume14
Issue number8
StatePublished - 1984
Externally publishedYes

Fingerprint

Immunoglobulin D
Germinal Center
Immune System
Antibodies
Injections
B-Lymphocytes
Lymph Nodes
Lymphocyte Activation
Immunoglobulin G
Peanut Agglutinin
Horseradish Peroxidase
Goats
Anti-Idiotypic Antibodies
Spleen
Cell Proliferation
Lymphocytes

ASJC Scopus subject areas

  • Immunology

Cite this

Polyclonal activation of the murine immune system by an antibody to IgD. V. Effect on germinal centers. / Flotte, Thomas J; Finkelman, F. D.; Thorbecke, G. J.

In: European Journal of Immunology, Vol. 14, No. 8, 1984, p. 725-728.

Research output: Contribution to journalArticle

@article{e3d8c4ed9831436eb33e4cdb8e556c60,
title = "Polyclonal activation of the murine immune system by an antibody to IgD. V. Effect on germinal centers",
abstract = "Heterologous anti-mouse δ chain has been shown to induce T-independent polyclonal B cell proliferation by 24 h after injection into mice and a T-dependent polyclonal increase in the number of IgG-secreting cells in spleen and lymph nodes 6 days after injection. The effect of anti-δ on germinal center cells has been difficult to determine, however, since anti-δ-induced blast transformation of mantle zone cells makes it difficult to distinguish these cells from germinal center cells. To clarify the effect of anti-δ on germinal centers, we stained lymph node sections from control and anti-δ-injected mice with peanut agglutinin conjugated to horseradish peroxidase which clearly differentiates the germinal center cells from other large and small lymphoid cells. For the first 5 days after i.v. injection of goat anti-mouse δ, the germinal center volumes did not differ significantly from those of mice injected with control antibodies. On days 6 and 7 after injection, the lymph node germinal center volumes of anti-δ-treated mice increased on average over 10-fold as compared to those of mice injected with an equal amount of control antibody. Thus, while anti-δ antibody directly induced blastogenesis of mantle layer B cells, it appears to have little direct effect on germinal center cells. In contrast, rapid germinal center development is seen in anti-δ-injected mice during the T-dependent phase of B cell activation and occurs simultaneously with the appearance of large numbers of IgG-secreting cells.",
author = "Flotte, {Thomas J} and Finkelman, {F. D.} and Thorbecke, {G. J.}",
year = "1984",
language = "English (US)",
volume = "14",
pages = "725--728",
journal = "European Journal of Immunology",
issn = "0014-2980",
publisher = "Wiley-VCH Verlag",
number = "8",

}

TY - JOUR

T1 - Polyclonal activation of the murine immune system by an antibody to IgD. V. Effect on germinal centers

AU - Flotte, Thomas J

AU - Finkelman, F. D.

AU - Thorbecke, G. J.

PY - 1984

Y1 - 1984

N2 - Heterologous anti-mouse δ chain has been shown to induce T-independent polyclonal B cell proliferation by 24 h after injection into mice and a T-dependent polyclonal increase in the number of IgG-secreting cells in spleen and lymph nodes 6 days after injection. The effect of anti-δ on germinal center cells has been difficult to determine, however, since anti-δ-induced blast transformation of mantle zone cells makes it difficult to distinguish these cells from germinal center cells. To clarify the effect of anti-δ on germinal centers, we stained lymph node sections from control and anti-δ-injected mice with peanut agglutinin conjugated to horseradish peroxidase which clearly differentiates the germinal center cells from other large and small lymphoid cells. For the first 5 days after i.v. injection of goat anti-mouse δ, the germinal center volumes did not differ significantly from those of mice injected with control antibodies. On days 6 and 7 after injection, the lymph node germinal center volumes of anti-δ-treated mice increased on average over 10-fold as compared to those of mice injected with an equal amount of control antibody. Thus, while anti-δ antibody directly induced blastogenesis of mantle layer B cells, it appears to have little direct effect on germinal center cells. In contrast, rapid germinal center development is seen in anti-δ-injected mice during the T-dependent phase of B cell activation and occurs simultaneously with the appearance of large numbers of IgG-secreting cells.

AB - Heterologous anti-mouse δ chain has been shown to induce T-independent polyclonal B cell proliferation by 24 h after injection into mice and a T-dependent polyclonal increase in the number of IgG-secreting cells in spleen and lymph nodes 6 days after injection. The effect of anti-δ on germinal center cells has been difficult to determine, however, since anti-δ-induced blast transformation of mantle zone cells makes it difficult to distinguish these cells from germinal center cells. To clarify the effect of anti-δ on germinal centers, we stained lymph node sections from control and anti-δ-injected mice with peanut agglutinin conjugated to horseradish peroxidase which clearly differentiates the germinal center cells from other large and small lymphoid cells. For the first 5 days after i.v. injection of goat anti-mouse δ, the germinal center volumes did not differ significantly from those of mice injected with control antibodies. On days 6 and 7 after injection, the lymph node germinal center volumes of anti-δ-treated mice increased on average over 10-fold as compared to those of mice injected with an equal amount of control antibody. Thus, while anti-δ antibody directly induced blastogenesis of mantle layer B cells, it appears to have little direct effect on germinal center cells. In contrast, rapid germinal center development is seen in anti-δ-injected mice during the T-dependent phase of B cell activation and occurs simultaneously with the appearance of large numbers of IgG-secreting cells.

UR - http://www.scopus.com/inward/record.url?scp=0021267625&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0021267625&partnerID=8YFLogxK

M3 - Article

C2 - 6332026

AN - SCOPUS:0021267625

VL - 14

SP - 725

EP - 728

JO - European Journal of Immunology

JF - European Journal of Immunology

SN - 0014-2980

IS - 8

ER -