Poly(-caprolactone)-carbon nanotube composite scaffolds for enhanced cardiac differentiation of human mesenchymal stem cells

Spencer W. Crowder, Yi Liang, Rutwik Rath, Andrew M. Park, Simon Maltais, Peter N. Pintauro, William Hofmeister, Chee C. Lim, Xintong Wang, Hak Joon Sung

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Aim: To evaluate the efficacy of electrically conductive, biocompatible composite scaffolds in modulating the cardiomyogenic differentiation of human mesenchymal stem cells (hMSCs). Materials & methods: Electrospun scaffolds of poly-caprolactone) with or without carbon nanotubes were developed to promote the in vitro cardiac differentiation of hMSCs. Results: Results indicate that hMSC differentiation can be enhanced by either culturing in electrically conductive, carbon nanotube-containing composite scaffolds without electrical stimulation in the presence of 5-azacytidine, or extrinsic electrical stimulation in nonconductive poly(-caprolactone) scaffolds without carbon nanotube and azacytidine. Conclusion: This study suggests a first step towards improving hMSC cardiomyogenic differentiation for local delivery into the infarcted myocardium.

Original languageEnglish (US)
Pages (from-to)1763-1776
Number of pages14
JournalNanomedicine
Volume8
Issue number11
DOIs
StatePublished - Nov 2013

Keywords

  • carbon nanotube
  • cardiac differentiation
  • electrical conductivity
  • mesenchymal stem cell
  • poly-caprolactone)

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

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    Crowder, S. W., Liang, Y., Rath, R., Park, A. M., Maltais, S., Pintauro, P. N., Hofmeister, W., Lim, C. C., Wang, X., & Sung, H. J. (2013). Poly(-caprolactone)-carbon nanotube composite scaffolds for enhanced cardiac differentiation of human mesenchymal stem cells. Nanomedicine, 8(11), 1763-1776. https://doi.org/10.2217/nnm.12.204