Pneumocystis carinii cell wall β-glucan induces release of macrophage inflammatory protein-2 from alveolar epithelial cells via a lactosylceramide-mediated mechanism

Peter Y. Hahn, Scott E. Evans, Theodore J. Kottom, Joseph E. Standing, Richard E. Pagano, Andrew Harold Limper

Research output: Contribution to journalArticle

110 Citations (Scopus)

Abstract

Infiltration of the lungs with neutrophils promotes respiratory failure during severe Pneumocystis carinii (PC) pneumonia. Recent studies have shown that alveolar epithelial cells (AECs), in addition to promoting PC attachment, also participate in lung inflammation by the release of cytokines and chemokines. Herein, we demonstrate that a PC β-glucan rich cell wall isolate (PCBG) stimulates the release of macrophage inflammatory protein-2 (MIP-2) from isolated AECs through a lactosylceramide-dependent mechanism. The results demonstrate that MIP-2 mRNA and protein production is significantly increased at both early and late time points after PCBG challenge. Although CD11b/CD18 (Mac-1, CR3) is the most widely studied β-glucan receptor, we demonstrate that CD11b/CD18 is not present on AECs. This study instead demonstrates that preincubation of AECs with an anti-body directed against the membrane glycosphingolipid lactosylceramide (CDw17) results in a significant decrease in MIP-2 secretion. Preincubation of the anti-CDw17 antibody with solubilized lactosylceramide reverses this effect. Furthermore, incubation of AECs with inhibitors of glycosphingolipid biosynthesis, including N-butyldeoxyno jirimycin and D-threo-1-phenyl-2-decanoylamino-3-morpholino-1-propanol-HC1, also results in a significant decrease in AEC MIP-2 production following challenge with PCBG. These data demonstrate that PC β-glucan induces significant production of MIP-2 from AECs and that CDw17 participates in the glucan-induced inflammatory signaling in lung epithelial cells during PC infection.

Original languageEnglish (US)
Pages (from-to)2043-2050
Number of pages8
JournalJournal of Biological Chemistry
Volume278
Issue number3
DOIs
StatePublished - Jan 17 2003

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Chemokine CXCL2
Alveolar Epithelial Cells
Pneumocystis carinii
Glucans
Cell Wall
Cells
Glycosphingolipids
Pneumocystis Infections
Lung
Pneumocystis Pneumonia
CDw17 antigen
Epithelial Cells
Chemokines
Biosynthesis
Respiratory Insufficiency
Infiltration
Anti-Idiotypic Antibodies
Pneumonia
Neutrophils
Cytokines

ASJC Scopus subject areas

  • Biochemistry

Cite this

Pneumocystis carinii cell wall β-glucan induces release of macrophage inflammatory protein-2 from alveolar epithelial cells via a lactosylceramide-mediated mechanism. / Hahn, Peter Y.; Evans, Scott E.; Kottom, Theodore J.; Standing, Joseph E.; Pagano, Richard E.; Limper, Andrew Harold.

In: Journal of Biological Chemistry, Vol. 278, No. 3, 17.01.2003, p. 2043-2050.

Research output: Contribution to journalArticle

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