Platelet phenol sulfotransferase and erythrocyte catechol-O-methyltransferase activities: Correlation with methyldopa metabolism

N. R C Campbell, J. H. Dunnette, G. Mwaluko, J. Van Loon, Richard M Weinshilboum

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Abstract

Methyldopa is metabolized by sulfate conjugation catalyzed by phenol sulfotransferase (PST), O-methylation catalyzed by catechol-O-methyltransferase (COMT), and decarboxylation catalyzed by aromatic l-amino acid decarboxylase. These experiments were performed to determine whether individual variations in red blood cell (RBC) COMT and platelet PST activities might reflect variations in the metabolism of methyldopa in man. Methyldopa, 3.5 mg/kg, was taken orally by 28 subjects. Blood samples were obtained from these subjects for the assay of platelet PST and RBC COMT activities, and a 24-hr urine sample was collected for the measurement of methyldopa and its major metabolites. Human platelets contain two independently regulated forms of PST. One form is thermolabile (TL), and the other is thermostable (TS). Methyldopa and α-methyldopamine are substrates for the TL but not for the TS form of PST. The results of the experiment showed significant correlations between TL platelet PST activity and the proportion of α-methyldopamine excreted as a sulfate conjugate, and between RBC COMT activity and the proportion of methyldopa excreted as an O-methyl metabolite. There was no significant correlation, however, between TL platelet PST activity, and the theportion of methyldopa itself excreted as a sulfate conjugate. These results are compatible with wit conclusion that differences among subjects in drug metabolizing enzyme activities are one factor responsible for wide individual variations in methyldopa metabolism in man.

Original languageEnglish (US)
Pages (from-to)55-63
Number of pages9
JournalClinical Pharmacology and Therapeutics
Volume35
Issue number1
StatePublished - Jan 1984

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Arylsulfotransferase
Methyldopa
Catechol O-Methyltransferase
Blood Platelets
Erythrocytes
Deoxyepinephrine
Sulfates
Aromatic-L-Amino-Acid Decarboxylases
Phenolsulfonphthalein
Wit and Humor
Decarboxylation
Methylation
Urine

ASJC Scopus subject areas

  • Pharmacology

Cite this

Platelet phenol sulfotransferase and erythrocyte catechol-O-methyltransferase activities : Correlation with methyldopa metabolism. / Campbell, N. R C; Dunnette, J. H.; Mwaluko, G.; Van Loon, J.; Weinshilboum, Richard M.

In: Clinical Pharmacology and Therapeutics, Vol. 35, No. 1, 01.1984, p. 55-63.

Research output: Contribution to journalArticle

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abstract = "Methyldopa is metabolized by sulfate conjugation catalyzed by phenol sulfotransferase (PST), O-methylation catalyzed by catechol-O-methyltransferase (COMT), and decarboxylation catalyzed by aromatic l-amino acid decarboxylase. These experiments were performed to determine whether individual variations in red blood cell (RBC) COMT and platelet PST activities might reflect variations in the metabolism of methyldopa in man. Methyldopa, 3.5 mg/kg, was taken orally by 28 subjects. Blood samples were obtained from these subjects for the assay of platelet PST and RBC COMT activities, and a 24-hr urine sample was collected for the measurement of methyldopa and its major metabolites. Human platelets contain two independently regulated forms of PST. One form is thermolabile (TL), and the other is thermostable (TS). Methyldopa and α-methyldopamine are substrates for the TL but not for the TS form of PST. The results of the experiment showed significant correlations between TL platelet PST activity and the proportion of α-methyldopamine excreted as a sulfate conjugate, and between RBC COMT activity and the proportion of methyldopa excreted as an O-methyl metabolite. There was no significant correlation, however, between TL platelet PST activity, and the theportion of methyldopa itself excreted as a sulfate conjugate. These results are compatible with wit conclusion that differences among subjects in drug metabolizing enzyme activities are one factor responsible for wide individual variations in methyldopa metabolism in man.",
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N2 - Methyldopa is metabolized by sulfate conjugation catalyzed by phenol sulfotransferase (PST), O-methylation catalyzed by catechol-O-methyltransferase (COMT), and decarboxylation catalyzed by aromatic l-amino acid decarboxylase. These experiments were performed to determine whether individual variations in red blood cell (RBC) COMT and platelet PST activities might reflect variations in the metabolism of methyldopa in man. Methyldopa, 3.5 mg/kg, was taken orally by 28 subjects. Blood samples were obtained from these subjects for the assay of platelet PST and RBC COMT activities, and a 24-hr urine sample was collected for the measurement of methyldopa and its major metabolites. Human platelets contain two independently regulated forms of PST. One form is thermolabile (TL), and the other is thermostable (TS). Methyldopa and α-methyldopamine are substrates for the TL but not for the TS form of PST. The results of the experiment showed significant correlations between TL platelet PST activity and the proportion of α-methyldopamine excreted as a sulfate conjugate, and between RBC COMT activity and the proportion of methyldopa excreted as an O-methyl metabolite. There was no significant correlation, however, between TL platelet PST activity, and the theportion of methyldopa itself excreted as a sulfate conjugate. These results are compatible with wit conclusion that differences among subjects in drug metabolizing enzyme activities are one factor responsible for wide individual variations in methyldopa metabolism in man.

AB - Methyldopa is metabolized by sulfate conjugation catalyzed by phenol sulfotransferase (PST), O-methylation catalyzed by catechol-O-methyltransferase (COMT), and decarboxylation catalyzed by aromatic l-amino acid decarboxylase. These experiments were performed to determine whether individual variations in red blood cell (RBC) COMT and platelet PST activities might reflect variations in the metabolism of methyldopa in man. Methyldopa, 3.5 mg/kg, was taken orally by 28 subjects. Blood samples were obtained from these subjects for the assay of platelet PST and RBC COMT activities, and a 24-hr urine sample was collected for the measurement of methyldopa and its major metabolites. Human platelets contain two independently regulated forms of PST. One form is thermolabile (TL), and the other is thermostable (TS). Methyldopa and α-methyldopamine are substrates for the TL but not for the TS form of PST. The results of the experiment showed significant correlations between TL platelet PST activity and the proportion of α-methyldopamine excreted as a sulfate conjugate, and between RBC COMT activity and the proportion of methyldopa excreted as an O-methyl metabolite. There was no significant correlation, however, between TL platelet PST activity, and the theportion of methyldopa itself excreted as a sulfate conjugate. These results are compatible with wit conclusion that differences among subjects in drug metabolizing enzyme activities are one factor responsible for wide individual variations in methyldopa metabolism in man.

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