Platelet lysate consisting of a natural repair proteome supports human mesenchymal stem cell proliferation and chromosomal stability

Ruben Crespo-Diaz, Atta Behfar, Greg W. Butler, Douglas J. Padley, Michael G. Sarr, Jozef Bartunek, Allan B Dietz, Andre Terzic

Research output: Contribution to journalArticle

126 Citations (Scopus)

Abstract

With favorable regenerative and immunotolerant profiles, patient-derived human mesenchymal stem cells (hMSCs) are increasingly considered in cell therapy. Derived from bone marrow (BM) and standardized with culture in fetal bovine serum (FBS), translation of hMSC-based approaches is impeded by protracted expansion times, risk of xenogenic response, and exposure to zoonoses. Here, human platelet lysate adherent to good manufacturing practices (GMP-hPL) provided a nonzoonotic adjuvant that enhanced the capacity of BM-hMSC to proliferate. The nurturing benefit of GMP-hPL was generalized to hMSC from adipose tissue evaluated as an alternative to bone marrow. Long-term culture in GMP-hPL maintained the multipotency of hMSC, while protecting against clonal chromosomal instability detected in the FBS milieu. Proteomic dissection identified TGF-β, VEGF, PDGF, FGF, and EGF as highly ranked effectors of hPL activity, revealing a paradigm of healing that underlies platelet lysate adjuvancy. Thus, GMP-adherent human platelet lysate accelerates hMSC proliferation with no chromosomal aberrancy, through an innate repair paradigm.

Original languageEnglish (US)
Pages (from-to)797-811
Number of pages15
JournalCell Transplantation
Volume20
Issue number6
DOIs
StatePublished - 2011

Fingerprint

Chromosomal Instability
Cell proliferation
Proteome
Platelets
Stem cells
Mesenchymal Stromal Cells
Repair
Blood Platelets
Cell Proliferation
Proteins
Bone
Bone Marrow
Dissection
Cell culture
Zoonoses
Cell- and Tissue-Based Therapy
Serum
Epidermal Growth Factor
Proteomics
Tissue

Keywords

  • Chromosomal stability
  • Human mesenchymal stem cells (hMSCs)
  • Human platelet lysate (hPL)

ASJC Scopus subject areas

  • Cell Biology
  • Transplantation
  • Biomedical Engineering

Cite this

Platelet lysate consisting of a natural repair proteome supports human mesenchymal stem cell proliferation and chromosomal stability. / Crespo-Diaz, Ruben; Behfar, Atta; Butler, Greg W.; Padley, Douglas J.; Sarr, Michael G.; Bartunek, Jozef; Dietz, Allan B; Terzic, Andre.

In: Cell Transplantation, Vol. 20, No. 6, 2011, p. 797-811.

Research output: Contribution to journalArticle

Crespo-Diaz, Ruben ; Behfar, Atta ; Butler, Greg W. ; Padley, Douglas J. ; Sarr, Michael G. ; Bartunek, Jozef ; Dietz, Allan B ; Terzic, Andre. / Platelet lysate consisting of a natural repair proteome supports human mesenchymal stem cell proliferation and chromosomal stability. In: Cell Transplantation. 2011 ; Vol. 20, No. 6. pp. 797-811.
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