Platelet-derived growth factor causes pulmonary cell proliferation and collagen deposition in vivo

Eunhee S. Yi, Hyesun Lee, Songmei Yin, Pierre Piguet, Ildiko Sarosi, Steve Kaufmann, John Tarpley, Nai San Wang, Thomas R. Ulich

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Platelet-derived growth factor (PDGF) is postulated to play a role in the pathophysiology of pulmonary fibrosis. Recombinant human PDGF-BB administered as a single intratracheal injection in rats causes an increase in peribronchial and perivascular stromal cells on days 2 and 3 after injection as evaluated by hematoxylin and eosin histology and 5- bromodeoxyuridine incorporation. Proliferation of bronchial epithelial cells and arterial smooth muscle cells, although not evident by routine histological examination alone, is detected on days 2 and 3 by increased 5- bromodeoxyuridine incorporation. A mild increase in 5-bromodeoxyuridine labeling is observed in peripheral alveolar parenchyma after injection of PDGF. The proliferative peribronchial and perivascular mesenchymal cells appear by light microscopic and ultrastructural criteria to be fibroblasts that are immunoreactive for vimentin but negative for α-smooth muscle actin and desmin. Daily intratracheal injection of PDGF-BB for 3 days causes a slightly more pronounced peribronchial and perivascular spindle cell proliferation accompanied by collagen deposition as evaluated by Masson's trichrome stain. PDGF-induced increases in cellularity and collagen resolve within 5 days after the last PDGF injection. In conclusion, intratracheal injection of PDGF-BB causes transient proliferation of pulmonary mesenchymal and epithelial cells accompanied by collagen deposition.

Original languageEnglish (US)
Pages (from-to)539-548
Number of pages10
JournalAmerican Journal of Pathology
Volume149
Issue number2
StatePublished - Aug 1996
Externally publishedYes

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Platelet-Derived Growth Factor
Collagen
Cell Proliferation
Lung
Injections
Bromodeoxyuridine
Epithelial Cells
Desmin
Pulmonary Fibrosis
Vimentin
Hematoxylin
Eosine Yellowish-(YS)
Stromal Cells
Smooth Muscle Myocytes
Smooth Muscle
Actins
Histology
Fibroblasts
Light
platelet-derived growth factor BB

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Yi, E. S., Lee, H., Yin, S., Piguet, P., Sarosi, I., Kaufmann, S., ... Ulich, T. R. (1996). Platelet-derived growth factor causes pulmonary cell proliferation and collagen deposition in vivo. American Journal of Pathology, 149(2), 539-548.

Platelet-derived growth factor causes pulmonary cell proliferation and collagen deposition in vivo. / Yi, Eunhee S.; Lee, Hyesun; Yin, Songmei; Piguet, Pierre; Sarosi, Ildiko; Kaufmann, Steve; Tarpley, John; Wang, Nai San; Ulich, Thomas R.

In: American Journal of Pathology, Vol. 149, No. 2, 08.1996, p. 539-548.

Research output: Contribution to journalArticle

Yi, ES, Lee, H, Yin, S, Piguet, P, Sarosi, I, Kaufmann, S, Tarpley, J, Wang, NS & Ulich, TR 1996, 'Platelet-derived growth factor causes pulmonary cell proliferation and collagen deposition in vivo', American Journal of Pathology, vol. 149, no. 2, pp. 539-548.
Yi ES, Lee H, Yin S, Piguet P, Sarosi I, Kaufmann S et al. Platelet-derived growth factor causes pulmonary cell proliferation and collagen deposition in vivo. American Journal of Pathology. 1996 Aug;149(2):539-548.
Yi, Eunhee S. ; Lee, Hyesun ; Yin, Songmei ; Piguet, Pierre ; Sarosi, Ildiko ; Kaufmann, Steve ; Tarpley, John ; Wang, Nai San ; Ulich, Thomas R. / Platelet-derived growth factor causes pulmonary cell proliferation and collagen deposition in vivo. In: American Journal of Pathology. 1996 ; Vol. 149, No. 2. pp. 539-548.
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