Platelet-derived exosomes induce cell proliferation and wound healing in human endometrial cells

Colleen M. Miller, Elizabeth Ann L Enninga, Skylar A. Rizzo, Jordan Phillipps, Hugo Guerrero-Cazares, Christopher C. Destephano, Timothy E. Peterson, Paul G. Stalboerger, Atta Behfar, Zaraq Khan

Research output: Contribution to journalArticlepeer-review

Abstract

Aim: To investigate the regenerative effects of a platelet-derived purified exosome product (PEP) on human endometrial cells. Materials & methods: Endometrial adenocarcinoma cells (HEC-1A), endometrial stromal cells (T HESC) and menstrual blood-derived stem cells (MenSC) were assessed for exosome absorption and subsequent changes in cell proliferation and wound healing properties over 48 h. Results: Cell proliferation increased in PEP treated T HESC (p < 0.0001) and MenSC (p < 0.001) after 6 h and in HEC-1A (p < 0.01) after 12 h. PEP improved wound healing after 6 h in HEC-1A (p < 0.01) and MenSC (p < 0.0001) and in T HESC between 24 and 36 h (p < 0.05). Conclusion: PEP was absorbed by three different endometrial cell types. PEP treatment increased cell proliferation and wound healing capacity.

Original languageEnglish (US)
Pages (from-to)805-817
Number of pages13
JournalRegenerative Medicine
Volume17
Issue number11
DOIs
StatePublished - Nov 1 2022

Keywords

  • endometrium
  • exosome
  • extracellular vesicle
  • menstrual blood-derived stem cells
  • platelet-derived exosomes

ASJC Scopus subject areas

  • Biomedical Engineering
  • Embryology

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