Platelet Counting by the RBC/Platelet Ratio Method: A Reference Method

George Klee; Guiseppe D'Onofrio; Onno W. Van Assendelft; Brian Bull; Ahnond Bunyaratvej; M. Buttarello; George Colella; Bruce Davis; Keiji Fujimoto; Warren Groner; Berend Houwen; Luc Van Hove; John A. Koepke; Mitchell Lewis; Sam Machin; Robert Raynor; Martin Rowan; Noriyuki Tatsumi; Kenneth A. Ault; Paul Harrison; Jolanta Kunicka; Francis Lacombe; Didier Lakomsky

doi:10.1309/W612-MYEP-FA7U-8UYA

Platelet Counting by the RBC/Platelet Ratio Method: A Reference Method

George Klee, Guiseppe D'Onofrio, Onno W. Van Assendelft, Brian Bull, Ahnond Bunyaratvej, M. Buttarello, George Colella, Bruce Davis, Keiji Fujimoto, Warren Groner, Berend Houwen, Luc Van Hove, John A. Koepke, Mitchell Lewis, Sam Machin, Robert Raynor, Martin Rowan, Noriyuki Tatsumi, Kenneth A. Ault, Paul HarrisonJolanta Kunicka, Francis Lacombe, Didier Lakomsky

Laboratory Medicine and Pathology

Research output: Contribution to journal › Article › peer-review

119 Scopus citations

Abstract

The International Council for Standardization in Haematology (ICSH) and the International Society of Laboratory Hematology (ISLH) recommend the counting of specifically labeled platelets relative to the RBCs with a fluorescence flow cytometer, together with an accurate RBC count determined with a semiautomated, single-channel aperture-impedance counter as a reference method for the enumeration of platelets. Fresh EDTA-anticoagulated venous blood specimens are measured within 4 hours of the draw. The specimen is prediluted (1:20) and the platelets labeled with two monoclonal antibodies specific to a cluster of differentiation common to all platelets. A final 1:1,000 dilution is made and at least 50,000 events with a minimum of 1,000 platelet events are counted with a flow cytometer to determine the RBC/platelet ratio. The platelet count is then calculated from this ratio and the RBC concentration of the original blood specimen.

Original language	English (US)
Pages (from-to)	460-464
Number of pages	5
Journal	American journal of clinical pathology
Volume	115
Issue number	3
DOIs	https://doi.org/10.1309/W612-MYEP-FA7U-8UYA
State	Published - Mar 2001

Keywords

Flow cytometry
Platelet count
Platelets
Reference method
Value assignment
Whole blood calibration

ASJC Scopus subject areas

Pathology and Forensic Medicine

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10.1309/W612-MYEP-FA7U-8UYA

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Klee, G., D'Onofrio, G., Van Assendelft, O. W., Bull, B., Bunyaratvej, A., Buttarello, M., Colella, G., Davis, B., Fujimoto, K., Groner, W., Houwen, B., Van Hove, L., Koepke, J. A., Lewis, M., Machin, S., Raynor, R., Rowan, M., Tatsumi, N., Ault, K. A., ... Lakomsky, D. (2001). Platelet Counting by the RBC/Platelet Ratio Method: A Reference Method. American journal of clinical pathology, 115(3), 460-464. https://doi.org/10.1309/W612-MYEP-FA7U-8UYA

Klee, G, D'Onofrio, G, Van Assendelft, OW, Bull, B, Bunyaratvej, A, Buttarello, M, Colella, G, Davis, B, Fujimoto, K, Groner, W, Houwen, B, Van Hove, L, Koepke, JA, Lewis, M, Machin, S, Raynor, R, Rowan, M, Tatsumi, N, Ault, KA, Harrison, P, Kunicka, J, Lacombe, F & Lakomsky, D 2001, 'Platelet Counting by the RBC/Platelet Ratio Method: A Reference Method', American journal of clinical pathology, vol. 115, no. 3, pp. 460-464. https://doi.org/10.1309/W612-MYEP-FA7U-8UYA

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abstract = "The International Council for Standardization in Haematology (ICSH) and the International Society of Laboratory Hematology (ISLH) recommend the counting of specifically labeled platelets relative to the RBCs with a fluorescence flow cytometer, together with an accurate RBC count determined with a semiautomated, single-channel aperture-impedance counter as a reference method for the enumeration of platelets. Fresh EDTA-anticoagulated venous blood specimens are measured within 4 hours of the draw. The specimen is prediluted (1:20) and the platelets labeled with two monoclonal antibodies specific to a cluster of differentiation common to all platelets. A final 1:1,000 dilution is made and at least 50,000 events with a minimum of 1,000 platelet events are counted with a flow cytometer to determine the RBC/platelet ratio. The platelet count is then calculated from this ratio and the RBC concentration of the original blood specimen.",

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AU - Bunyaratvej, Ahnond

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AU - Machin, Sam

AU - Raynor, Robert

AU - Rowan, Martin

AU - Tatsumi, Noriyuki

AU - Ault, Kenneth A.

AU - Harrison, Paul

AU - Kunicka, Jolanta

AU - Lacombe, Francis

AU - Lakomsky, Didier

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AB - The International Council for Standardization in Haematology (ICSH) and the International Society of Laboratory Hematology (ISLH) recommend the counting of specifically labeled platelets relative to the RBCs with a fluorescence flow cytometer, together with an accurate RBC count determined with a semiautomated, single-channel aperture-impedance counter as a reference method for the enumeration of platelets. Fresh EDTA-anticoagulated venous blood specimens are measured within 4 hours of the draw. The specimen is prediluted (1:20) and the platelets labeled with two monoclonal antibodies specific to a cluster of differentiation common to all platelets. A final 1:1,000 dilution is made and at least 50,000 events with a minimum of 1,000 platelet events are counted with a flow cytometer to determine the RBC/platelet ratio. The platelet count is then calculated from this ratio and the RBC concentration of the original blood specimen.

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Platelet Counting by the RBC/Platelet Ratio Method: A Reference Method

Abstract

Keywords

ASJC Scopus subject areas

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