TY - JOUR
T1 - Platelet characteristics change with aging
T2 - Role of estrogen receptor β
AU - Jayachandran, Muthuvel
AU - Karnicki, Krzysztof
AU - Miller, Randall S.
AU - Owen, Whyte G.
AU - Korach, Kenneth S.
AU - Miller, Virginia M.
N1 - Funding Information:
ACKNOWLEDGMENTS This work was supported by National Institutes of Health Grant HL51736 and by the Mayo Foundation.
PY - 2005/7
Y1 - 2005/7
N2 - Estrogen receptor beta (βER) is the predominant estrogen receptor in platelets. Experiments were designed to define phenotypic changes in platelets with aging following deletion of βER (βERKO). Blood was collected from wild-type and βERKO female mice at 4-7 (young) and 24-25 (aged) months of age. In young animals, total number of platelets, number of platelets containing RNA (reticulated platelets), aggregation, dense body adenosine triphosphate secretion, and alpha granular secretion were the same in both groups. With aging, total number of platelets decreased but reticulated platelets increased in βERKO mice; aggregation and dense granule adenosine triphosphate secretion decreased whereas basal expression of fibrinogen receptors increased with age in wild-type and βERKO mice. Basal expression of P-selectin and annexin V binding increased with aging only in βERKO mice; thrombin did not increase expression in these mice. Therefore, deletion of βER is associated with specific platelet functions, which are expressed only with age-associated reproductive senescence.
AB - Estrogen receptor beta (βER) is the predominant estrogen receptor in platelets. Experiments were designed to define phenotypic changes in platelets with aging following deletion of βER (βERKO). Blood was collected from wild-type and βERKO female mice at 4-7 (young) and 24-25 (aged) months of age. In young animals, total number of platelets, number of platelets containing RNA (reticulated platelets), aggregation, dense body adenosine triphosphate secretion, and alpha granular secretion were the same in both groups. With aging, total number of platelets decreased but reticulated platelets increased in βERKO mice; aggregation and dense granule adenosine triphosphate secretion decreased whereas basal expression of fibrinogen receptors increased with age in wild-type and βERKO mice. Basal expression of P-selectin and annexin V binding increased with aging only in βERKO mice; thrombin did not increase expression in these mice. Therefore, deletion of βER is associated with specific platelet functions, which are expressed only with age-associated reproductive senescence.
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U2 - 10.1093/gerona/60.7.815
DO - 10.1093/gerona/60.7.815
M3 - Article
C2 - 16079202
AN - SCOPUS:23244453963
SN - 1079-5006
VL - 60
SP - 815
EP - 819
JO - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
JF - Journals of Gerontology - Series A Biological Sciences and Medical Sciences
IS - 7
ER -