Plasmonic nanopore arrays for characterizing the binding of myelin growth promoting igm antibodies to supported lipid bilayers

Nathan J. Wittenberg, Hyungsoon Im, Arthur E. Warrington, Moses Rodriguez, Sang Hyun Oh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We characterized IgM antibody binding to supported lipid bilayers (SLBs) on gold plasmonic nanopores using surface plasmon resonance (SPR) sensing. The antibodies studied, IgMO1 and IgMO4 bind sulfatide (Sulf) and galactocerebroside (GalC), respectively, and initiate myelin regrowth in mouse models of multiple sclerosis. SLBs containing Sulf and GalC are formed on nanopore arrays inside PDMS microfluidics. The optical transmission spectrum through the nanopore array is sensitive to local refractive index and red shifts as antibodies bind to the lipid bilayer. Analysis of the association and dissociation curves allows calculation of dissociation constants (KD) for IgMO4/Sulf and IgMO1/GalC binding.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1089-1091
Number of pages3
StatePublished - Dec 1 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Publication series

Name15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Volume2

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

Keywords

  • Antibodies
  • Nanopores
  • Supported lipid bilayer
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Control and Systems Engineering

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  • Cite this

    Wittenberg, N. J., Im, H., Warrington, A. E., Rodriguez, M., & Oh, S. H. (2011). Plasmonic nanopore arrays for characterizing the binding of myelin growth promoting igm antibodies to supported lipid bilayers. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (pp. 1089-1091). (15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011; Vol. 2).