TY - GEN
T1 - Plasmonic nanopore arrays for characterizing the binding of myelin growth promoting igm antibodies to supported lipid bilayers
AU - Wittenberg, Nathan J.
AU - Im, Hyungsoon
AU - Warrington, Arthur E.
AU - Rodriguez, Moses
AU - Oh, Sang Hyun
N1 - Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2011
Y1 - 2011
N2 - We characterized IgM antibody binding to supported lipid bilayers (SLBs) on gold plasmonic nanopores using surface plasmon resonance (SPR) sensing. The antibodies studied, IgMO1 and IgMO4 bind sulfatide (Sulf) and galactocerebroside (GalC), respectively, and initiate myelin regrowth in mouse models of multiple sclerosis. SLBs containing Sulf and GalC are formed on nanopore arrays inside PDMS microfluidics. The optical transmission spectrum through the nanopore array is sensitive to local refractive index and red shifts as antibodies bind to the lipid bilayer. Analysis of the association and dissociation curves allows calculation of dissociation constants (KD) for IgMO4/Sulf and IgMO1/GalC binding.
AB - We characterized IgM antibody binding to supported lipid bilayers (SLBs) on gold plasmonic nanopores using surface plasmon resonance (SPR) sensing. The antibodies studied, IgMO1 and IgMO4 bind sulfatide (Sulf) and galactocerebroside (GalC), respectively, and initiate myelin regrowth in mouse models of multiple sclerosis. SLBs containing Sulf and GalC are formed on nanopore arrays inside PDMS microfluidics. The optical transmission spectrum through the nanopore array is sensitive to local refractive index and red shifts as antibodies bind to the lipid bilayer. Analysis of the association and dissociation curves allows calculation of dissociation constants (KD) for IgMO4/Sulf and IgMO1/GalC binding.
KW - Antibodies
KW - Nanopores
KW - Supported lipid bilayer
KW - Surface plasmon resonance
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M3 - Conference contribution
AN - SCOPUS:84883776656
SN - 9781618395955
T3 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
SP - 1089
EP - 1091
BT - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
T2 - 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Y2 - 2 October 2011 through 6 October 2011
ER -