Plasmonic nanopore arrays for characterizing the binding of myelin growth promoting igm antibodies to supported lipid bilayers

Nathan J. Wittenberg, Hyungsoon Im, Arthur E. Warrington, Moses Rodriguez, Sang Hyun Oh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We characterized IgM antibody binding to supported lipid bilayers (SLBs) on gold plasmonic nanopores using surface plasmon resonance (SPR) sensing. The antibodies studied, IgMO1 and IgMO4 bind sulfatide (Sulf) and galactocerebroside (GalC), respectively, and initiate myelin regrowth in mouse models of multiple sclerosis. SLBs containing Sulf and GalC are formed on nanopore arrays inside PDMS microfluidics. The optical transmission spectrum through the nanopore array is sensitive to local refractive index and red shifts as antibodies bind to the lipid bilayer. Analysis of the association and dissociation curves allows calculation of dissociation constants (KD) for IgMO4/Sulf and IgMO1/GalC binding.

Original languageEnglish (US)
Title of host publication15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
Pages1089-1091
Number of pages3
Volume2
StatePublished - 2011
Event15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 - Seattle, WA, United States
Duration: Oct 2 2011Oct 6 2011

Other

Other15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011
CountryUnited States
CitySeattle, WA
Period10/2/1110/6/11

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Lipid bilayers
Nanopores
Antibodies
Surface plasmon resonance
Light transmission
Microfluidics
Refractive index
Gold
Association reactions

Keywords

  • Antibodies
  • Nanopores
  • Supported lipid bilayer
  • Surface plasmon resonance

ASJC Scopus subject areas

  • Control and Systems Engineering

Cite this

Wittenberg, N. J., Im, H., Warrington, A. E., Rodriguez, M., & Oh, S. H. (2011). Plasmonic nanopore arrays for characterizing the binding of myelin growth promoting igm antibodies to supported lipid bilayers. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011 (Vol. 2, pp. 1089-1091)

Plasmonic nanopore arrays for characterizing the binding of myelin growth promoting igm antibodies to supported lipid bilayers. / Wittenberg, Nathan J.; Im, Hyungsoon; Warrington, Arthur E.; Rodriguez, Moses; Oh, Sang Hyun.

15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2 2011. p. 1089-1091.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Wittenberg, NJ, Im, H, Warrington, AE, Rodriguez, M & Oh, SH 2011, Plasmonic nanopore arrays for characterizing the binding of myelin growth promoting igm antibodies to supported lipid bilayers. in 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. vol. 2, pp. 1089-1091, 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011, Seattle, WA, United States, 10/2/11.
Wittenberg NJ, Im H, Warrington AE, Rodriguez M, Oh SH. Plasmonic nanopore arrays for characterizing the binding of myelin growth promoting igm antibodies to supported lipid bilayers. In 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2. 2011. p. 1089-1091
Wittenberg, Nathan J. ; Im, Hyungsoon ; Warrington, Arthur E. ; Rodriguez, Moses ; Oh, Sang Hyun. / Plasmonic nanopore arrays for characterizing the binding of myelin growth promoting igm antibodies to supported lipid bilayers. 15th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2011, MicroTAS 2011. Vol. 2 2011. pp. 1089-1091
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