Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment

Antoine Lesuffleur, Kwan Seop Lim, Nathan C. Lindquist, Hyungsoon Im, Arthur E. Warrington, Moses Rodriguez, Sang Hyun Oh

Research output: Chapter in Book/Report/Conference proceedingConference contribution

8 Citations (Scopus)

Abstract

We integrate periodic nanohole arrays in a thin gold film with lipid membranes in a microfluidic channel. Surface plasmon-enhanced light transmission through the periodic nanohole arrays enables real-time label-free sensing of molecular binding on the lipid membrane surface. This membrane biosensor can potentially act as a natural platform for studying binding kinetics of proteins with their binding partners anchored in the lipid membrane. We also present the concept of using nanopore arrays for kinetic assays of transmembrane proteins in a free-standing lipid membrane.

Original languageEnglish (US)
Title of host publicationProceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009
Pages1481-1484
Number of pages4
DOIs
StatePublished - 2009
Event31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009 - Minneapolis, MN, United States
Duration: Sep 2 2009Sep 6 2009

Other

Other31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009
CountryUnited States
CityMinneapolis, MN
Period9/2/099/6/09

Fingerprint

Membrane Lipids
Labels
Kinetics
Nanopores
Proteins
Microfluidics
Biosensing Techniques
Light transmission
Biosensors
Gold
Assays
Carrier Proteins
Membranes
Light

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Biomedical Engineering
  • Medicine(all)

Cite this

Lesuffleur, A., Lim, K. S., Lindquist, N. C., Im, H., Warrington, A. E., Rodriguez, M., & Oh, S. H. (2009). Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment. In Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009 (pp. 1481-1484). [5332429] https://doi.org/10.1109/IEMBS.2009.5332429

Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment. / Lesuffleur, Antoine; Lim, Kwan Seop; Lindquist, Nathan C.; Im, Hyungsoon; Warrington, Arthur E.; Rodriguez, Moses; Oh, Sang Hyun.

Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009. 2009. p. 1481-1484 5332429.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Lesuffleur, A, Lim, KS, Lindquist, NC, Im, H, Warrington, AE, Rodriguez, M & Oh, SH 2009, Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment. in Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009., 5332429, pp. 1481-1484, 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009, Minneapolis, MN, United States, 9/2/09. https://doi.org/10.1109/IEMBS.2009.5332429
Lesuffleur A, Lim KS, Lindquist NC, Im H, Warrington AE, Rodriguez M et al. Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment. In Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009. 2009. p. 1481-1484. 5332429 https://doi.org/10.1109/IEMBS.2009.5332429
Lesuffleur, Antoine ; Lim, Kwan Seop ; Lindquist, Nathan C. ; Im, Hyungsoon ; Warrington, Arthur E. ; Rodriguez, Moses ; Oh, Sang Hyun. / Plasmonic nanohole arrays for label-free kinetic biosensing in a lipid membrane environment. Proceedings of the 31st Annual International Conference of the IEEE Engineering in Medicine and Biology Society: Engineering the Future of Biomedicine, EMBC 2009. 2009. pp. 1481-1484
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