TY - JOUR
T1 - Plasmacytoid dendritic cells protect against atherosclerosis by tuning T-cell proliferation and activity
AU - Daissormont, Isabelle T.M.N.
AU - Christ, Anette
AU - Temmerman, Lieve
AU - Millares, Stefan Sampedro
AU - Seijkens, Tom
AU - Manca, Marco
AU - Rousch, Mat
AU - Poggi, Marjorie
AU - Boon, Louis
AU - Van Der Loos, Chris
AU - Daemen, Mat
AU - Lutgens, Esther
AU - Halvorsen, Bente
AU - Aukrust, Pal
AU - Janssen, Edith
AU - Biessen, Erik A.L.
PY - 2011/12/9
Y1 - 2011/12/9
N2 - Rationale: Unlike conventional dendritic cells, plasmacytoid DCs (PDC) are poor in antigen presentation and critical for type I interferon response. Though proposed to be present in human atherosclerotic lesions, their role in atherosclerosis remains elusive. Objective: To investigate the role of PDC in atherosclerosis. Methods and Results: We show that PDC are scarcely present in human atherosclerotic lesions and almost absent in mouse plaques. Surprisingly, PDC depletion by 120G8 mAb administration was seen to promote plaque T-cell accumulation and exacerbate lesion development and progression in LDLr -/- mice. PDC depletion was accompanied by increased CD4 + T-cell proliferation, interferon-γ expression by splenic T cells, and plasma interferon-γ levels. Lymphoid tissue PDC from atherosclerotic mice showed increased indoleamine 2,3-dioxygenase (IDO) expression and IDO blockage abrogated the PDC suppressive effect on T-cell proliferation. Conclusions: Our data reveal a protective role for PDC in atherosclerosis, possibly by dampening T-cell proliferation and activity in peripheral lymphoid tissue, rendering PDC an interesting target for future therapeutic interventions.
AB - Rationale: Unlike conventional dendritic cells, plasmacytoid DCs (PDC) are poor in antigen presentation and critical for type I interferon response. Though proposed to be present in human atherosclerotic lesions, their role in atherosclerosis remains elusive. Objective: To investigate the role of PDC in atherosclerosis. Methods and Results: We show that PDC are scarcely present in human atherosclerotic lesions and almost absent in mouse plaques. Surprisingly, PDC depletion by 120G8 mAb administration was seen to promote plaque T-cell accumulation and exacerbate lesion development and progression in LDLr -/- mice. PDC depletion was accompanied by increased CD4 + T-cell proliferation, interferon-γ expression by splenic T cells, and plasma interferon-γ levels. Lymphoid tissue PDC from atherosclerotic mice showed increased indoleamine 2,3-dioxygenase (IDO) expression and IDO blockage abrogated the PDC suppressive effect on T-cell proliferation. Conclusions: Our data reveal a protective role for PDC in atherosclerosis, possibly by dampening T-cell proliferation and activity in peripheral lymphoid tissue, rendering PDC an interesting target for future therapeutic interventions.
KW - Atherosclerosis
KW - Immune tolerance
KW - Plasmacytoid dendritic cells
KW - T cells
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U2 - 10.1161/CIRCRESAHA.111.256529
DO - 10.1161/CIRCRESAHA.111.256529
M3 - Article
C2 - 22021930
AN - SCOPUS:84856096476
SN - 0009-7330
VL - 109
SP - 1387
EP - 1395
JO - Circulation research
JF - Circulation research
IS - 12
ER -